Mouse Monoclonal IgG2a Anti-Surfactant Protein B-Precursor (Pro-SP-B) [SPB01 (1-2-A5-9)]

• Catalog number
  SPB001
• Price
  Please ask
• Size
  1 ml

• Availability
  Please contact us to check the availability
• Immunogen
  Recombinant Pro-Surfactant Protein B
• Specificity
  Pro-Surfactant Protein B, no reactivity with mature SPB
• Species reactivity
  Human, Rat, others not tested
• Product presentation
  Antibody solution in stabilizing phosphate buffer pH 7.3. Contains 0.09 % sodium azide**. The volume is sufficient for at least 100 immunohistochemical tests (100 µl working solution / test). Use appropriate antibody diluent e.g. Gentaur Art .No. PU002.
• Product category
  Primary Antibody
• Antibody s raised in
  Mouse
• Antibody s isotype
  IgG2a
• Antibody s clone
  SPB01 (1-2-A5-9)
• Product is supplied as
  liquid concentrate
• Recommended concentration for use
  2-4 µg/ml
• Protein concentration
  50 µg/ml
• Positive control
  Lung adenocarcinoma
• Pretreatment
  After formaldehyde fixation antigen unmasking at 96-100°C is recommended (use citrate buffer Art. No. DE000 or Unmasking Fluid G on glycol basis Art. No. DE007 respectively).
• Tested applications
  IHC(C, P), IFA
• Recommended secondary reagent
  As secondary systems in immunohistochemistry we recommend the VECTASTAIN Elite ABC Mouse IgG system (Art. No. PK-6102) or VECTOR ImmPress Mouse IgG (Art. No. MP-7402) and Gentaur Universal Staining Kits DAB and AEC (Art. No. DA005 and AE005).
• Storage method
  Store at 2-8°C
• UniProt number
  Go to UniProt/SwissProt
• Litterature
  1. Zhou L., Lim L., Costa R.H., and Whitsett J.A. (1996) Thyroid transcription factor-1, hepatocyte nuclear factor-3beta, surfactant protein B, C, and Clara cell secretory protein in developing mouse lung. J. Histochem. And Cytochem. 44(10);1183-1193. 2. Khoor A. ; Whitsett J. A. ; Stahlman M. T. ; Olson S. J. ; Cagle P. T. (1999) Utility of surfactant protein B precursor and thyroid transcription factor 1 in differentiating adenocarcinoma of the lung from malignant mesothelioma Human pathology 30(6);695-700. 3. Korimilli A., Gonzales L.W., and Guttentag S.H. (2000) Intracellular Localization of Processing Events in Human Surfactant Protein B Biosynthesis* J Biol Chem, 275(12); 8672-8679. 4. Kolla V., Gonzales L.W., Gonzales J., Wang P., Angampalli S., Feinstein S.I., and Ballard P. L. (2007) Thyroid Transcription Factor in Differentiating Type II Cells: Regulation, Isoforms, and Target Genes Am. J. Respir. Cell Mol. Biol.36(2): 213 - 225.
• Tips
  *Gentaur's antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals. ** The preservative sodium azide is known to be poisonous and potentially hazardous to health. It should be handled only by trained staff. Despite of the product's low azide concentration it must be handled with care. Dispose according to regional rules!
• Other products
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use. Precursor cell, also called a blast cell or simply blast, is a type of partially differentiated, usually unipotent cell that has lost most or all of the stem cell multipotency.
• About
  Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
• Test
  Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
• Latin name
  Mus musculus

• Gene target
  Monoclonal   IgG2a   Surfactant   Protein   B-Precursor   Pro-SP-B   [SPB01   1-2-A5-9  
• Gene symbol
  SFTPB, SPATA1, PCDHGA5, SFTPA1, LINC00570, MIR9-2, ERVPABLB-1, MIR9-1, BATF, CSH2
• Short name
  Research
• Technique
  Mouse monoclonal, anti-, Mouse, anti, antibody to, antibodies, Monoclonals or monoclonal antibodies, mouses, mouse monoclonals
• Host
  mouse monoclonal, Murine monoclonal antibodies or Mabs are often conjugated and the isotype is IgG.
• Isotype
  IgG2a
• Species
  Mouse, Mouses
• Alternative name
  Surfactant Protein B-Precursor (Pro-SP-B)
• Alternative technique
  murine, antibodies

Gene info

• Identity
  HGNC:10801
• Gene
  SFTPB
• Long gene name
  surfactant protein B
• Synonyms gene
  • SFTP3
• Synonyms gene name
  • surfactant, pulmonary-associated protein B
• Synonyms
  • SP-B
• GenBank acession
  • J02761
• Locus
  2p11.2
• Discovery year
  1988-07-06
• Entrez gene record
  6439
• Pubmed identfication
  • 2924687
  • 1346779
• RefSeq identity
  • NM_198843
• VEGA ID
  OTTHUMG00000130181

Gene info

• Identity
  HGNC:14682
• Gene
  SPATA1
• Long gene name
  spermatogenesis associated 1
• Synonyms
  • SP-2
• GenBank acession
  • AF306347
• Locus
  1p22.3
• Discovery year
  2001-03-01
• Entrez gene record
  100505741
• RefSeq identity
  • NM_022354
• VEGA ID
  OTTHUMG00000009924

Gene info

• Identity
  HGNC:8703
• Gene
  PCDHGA5
• Long gene name
  protocadherin gamma subfamily A, 5
• Synonyms
  • CDH-GAMMA-A5
  • ME3
  • PCDH-GAMMA-A5
• Synonyms name
  • cadherin ME3
• GenBank acession
  • AF152512
• Locus
  5q31
• Discovery year
  2000-06-28
• Entrez gene record
  56110
• Pubmed identfication
  • 10380929
• RefSeq identity
  • NM_018918
• Classification
  • Clustered protocadherins
• VEGA ID
  OTTHUMG00000163683

Gene info

• Identity
  HGNC:10798
• Gene
  SFTPA1
• Long gene name
  surfactant protein A1
• Synonyms gene
  • SFTP1
• Synonyms gene name
  • surfactant, pulmonary-associated protein A1
• Synonyms
  • SP-A
  • SP-A1
  • COLEC4
• Synonyms name
  • surfactant, pulmonary-associated protein A1A
• GenBank acession
  • BC026229
• Locus
  10q22.3
• Discovery year
  2001-06-22
• Entrez gene record
  653509
• RefSeq identity
  • NM_005411
• Classification
  • Collectins
  • C-type lectin domain containing
• VEGA ID
  OTTHUMG00000018565

Gene info

• Identity
  HGNC:43717
• Gene
  LINC00570
• Long gene name
  long intergenic non-protein coding RNA 570
• Synonyms
  • ncRNA-a5
• GenBank acession
  • BX102688
  • AI493106
• Locus
  2p25.1
• Discovery year
  2012-02-02
• Pubmed identfication
  • 20887892
• Classification
  • Long intergenic non-protein coding RNAs
• VEGA ID
  OTTHUMG00000151818

Gene info

• Identity
  HGNC:31642
• Gene
  MIR9-2
• Long gene name
  microRNA 9-2
• Synonyms gene
  • MIRN9-2
• Synonyms
  • hsa-mir-9-2
• Locus
  5q14.3
• Discovery year
  2004-04-23
• Entrez gene record
  407047
• RefSeq identity
  • NR_030741
• Classification
  • MicroRNA MIR9 family

Gene info

• Identity
  HGNC:39042
• Gene
  ERVPABLB-1
• Long gene name
  endogenous retrovirus group PABLB member 1, envelope
• Synonyms gene name
  • endogenous retrovirus group PABLB, member 1
  • endogenous retrovirus group PABLB member 1
• Synonyms
  • envR(b)
• Synonyms name
  • HERV-R(b)_3p24.3 provirus ancestral Env polyprotein
• GenBank acession
  • BQ012865
  • CF529244
  • AI189490
• Locus
  3p24.3
• Discovery year
  2011-05-05
• Entrez gene record
  100862693
• Pubmed identfication
  • 12970426
  • 21542922
• Classification
  • Envelope ERV derived genes

Gene info

• Identity
  HGNC:31641
• Gene
  MIR9-1
• Long gene name
  microRNA 9-1
• Synonyms gene
  • MIRN9-1
• Synonyms
  • hsa-mir-9-1
• Locus
  1q22
• Discovery year
  2004-04-23
• Entrez gene record
  407046
• RefSeq identity
  • NR_029691
• Classification
  • MicroRNA MIR9 family

Gene info

• Identity
  HGNC:958
• Gene
  BATF
• Long gene name
  basic leucine zipper ATF-like transcription factor
• Synonyms gene name
  • basic leucine zipper transcription factor, ATF-like
• Synonyms
  • B-ATF
  • SFA-2
  • BATF1
• Synonyms name
  • activating transcription factor B
  • SF-HT-activated gene 2
• GenBank acession
  • AF016898
• Locus
  14q24.3
• Discovery year
  2000-05-15
• Entrez gene record
  10538
• Pubmed identfication
  • 8570175
  • 8630063
• RefSeq identity
  • NM_006399
• Classification
  • Basic leucine zipper proteins
• VEGA ID
  OTTHUMG00000171486

Gene info

• Identity
  HGNC:2441
• Gene
  CSH2
• Long gene name
  chorionic somatomammotropin hormone 2
• Synonyms
  • hCS-B
  • CSB
  • CS-2
• Synonyms name
  • placental lactogen
  • chorionic somatomammotropin B
• GenBank acession
  • V00573
• Locus
  17q23.3
• Discovery year
  2001-06-22
• Entrez gene record
  1443
• Pubmed identfication
  • 593368
  • 6208192
• RefSeq identity
  • NM_020991
• Classification
  • Growth hormone family
• VEGA ID
  OTTHUMG00000172283

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

Product images

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