Mouse Monoclonal IgG2a Anti-human CD20 [L26]
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Catalog numberCD20-1
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PricePlease ask
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Size1 ml
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AvailabilityPlease contact us to check the availability
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ImmunogenB-Cells from human Tonsils
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SpecificityHuman CD20cy on peripheral and lymphoidal B-cells, germ center of lymph knots.
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Species reactivityHuman
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Product presentationAntibody solution in stabilizing phosphate buffer pH 7.3. Contains 0.09 % sodium azide**. The volume is sufficient for at least 100 immunohistochemical tests (100 µl working solution / test). Use appropriate antibody diluent e.g. Gentaur Art .No. PU002.
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Product categoryPrimary Antibody
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Antibody s raised inMouse
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Antibody s isotypeIgG2a
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Antibody s cloneL26
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Product is supplied asliquid concentrate
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Recommended concentration for use1:10 to 1:50
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Protein concentrationN/A
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Positive controlLymph nodes, Tonsil
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PretreatmentImprovement of staining with Unmasking Fluid G (Art. No. DE007) or Unmasking Fluid C (Art. No. DE000)
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Tested applicationsIHC(C, P), FACS
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Recommended secondary reagentWe recommend the use of Gentaur's Universal Staining System DAB (Art. No. DA005) or AEC (Art.-No. AE005), if higher sensitivity is required VECTASTAIN Elite ABC (Art. No. PK-6102) or ABC AP (Art. No. AK-5002) systems are applicable.
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Storage methodStore at 2-8°C
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UniProt numberGo to UniProt/SwissProt
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Litterature1. Zhou l.-Z. and Tedder T. (1993) CD20 Workshop panel report. In Schlossman S.F. et al. Eds. Leucocyte Typing V. White cell differentiation antigens. Oxford University Press, Oxford, N.Y., Tokyo; pp 511-514. 2. Takami T., Qi C., Yamada T., et al. (1989) B20.3. Reactivity and Specificity of L26 (pan-B-cell mAb) on 322 cases of fresh and paraffin-embedded lymphoproliferative diseases. In Kapp W. et al. eds. Leucocyte Typing IV. White Cell Differentiation Antigens. Oxford University Press, Oxford, N.Y., Tokyo; pp 134-136. 3. Mason D.Y., Comans-Bitter WM., Cordell J.L., Verhoeven M.A.J. and van Dongen J.J.M. (1990) Antibody L26 recognizes an intracellular epitope of the B-cell-associated CD20 antigen. Am. J. Pathol. 136; 1215-1222.
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Tips*Gentaur's antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals. ** The preservative sodium azide is known to be poisonous and potentially hazardous to health. It should be handled only by trained staff. Despite of the product's low azide concentration it must be handled with care. Dispose according to regional rules!
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Testbio logo supplies antibodies that are for research of human proteins. Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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PropertiesHuman proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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AboutMonoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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Latin nameMus musculus
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Gene target
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Gene symbolRPL26, MRPL17, MS4A1
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Short nameBioprime
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TechniqueMouse monoclonal, anti-Human, anti-, Mouse, anti, antibody to, antibodies, antibodies against human proteins, Monoclonals or monoclonal antibodies, mouses, mouse monoclonals
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Hostmouse monoclonal, Murine monoclonal antibodies or Mabs are often conjugated and the isotype is IgG.
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IsotypeIgG2a
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SpeciesHuman, Humans, Mouses
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Alternative nameCD20 (L26), B Cells, human
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Alternative techniquemurine, antibodies
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Gene info
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Identity
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Gene
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Long gene nameribosomal protein L26
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Synonyms
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Locus
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Discovery year1993-06-15
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- L ribosomal proteins
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VEGA ID
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Locus Specific Databases
Gene info
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Identity
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Gene
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Long gene namemitochondrial ribosomal protein L17
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Synonyms
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GenBank acession
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Locus
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Discovery year2001-02-28
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Entrez gene record
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RefSeq identity
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Classification
- Large subunit mitochondrial ribosomal proteins
- Mitochondrial ribosomal proteins
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namemembrane spanning 4-domains A1
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Synonyms gene
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Synonyms gene name
- membrane-spanning 4-domains, subfamily A, member 1
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Synonyms
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GenBank acession
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Locus
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Discovery year1989-05-23
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Entrez gene record
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Pubmed identfication
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Classification
- Membrane spanning 4-domains
- CD molecules
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VEGA ID
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Locus Specific Databases
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data