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Availability
Please contact us to check the availability
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Immunogen
Purified Antigen from Thymocyte and T-cell extract
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Specificity
Human CD4 molecule, D1 domain
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Species reactivity
Human
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Product presentation
Cell culture medium with phosphate buffer, BSA and 0.09 % sodium azide**.
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Product category
Primary Antibody
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Antibody s raised in
Mouse
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Antibody s isotype
IgG2a
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Antibody s clone
MEM115
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Product is supplied as
ready to use
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Recommended concentration for use
neat
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Protein concentration
N/A
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Positive control
Tonsil
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Pretreatment
The antigen epitope detected by clone MEM-115 may be sensitive to the treatment with H2O2. Therefore it is recommended to block endogenous peroxidases with 0.5% H2O2 in Methanol (10 min incubation). This step should be carried out prior to antigen unmasking! Antigen unmasking is performed at 96-100° Celsius in 1 mM EDTA pH 8.0 for 10-20 min.
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Tested applications
IHC(C, P)
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Recommended secondary reagent
We recommend the use of Gentaur's Universal Staining System DAB (Art. No. DA005) or AEC (Art.-No. AE005), if higher sensitivity is required VECTASTAIN Elite ABC (Art. No. PK-6102) or ABC AP (Art. No. AK-5002) systems are applicable.
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Storage method
Store at 2-8°C
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UniProt number
PGo to UniProt/SwissProt173Go to UniProt/SwissProt (CD4_HUMAN)
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Litterature
1. Falini B, Stein H, Pileri S, Canino S, Farabbi R, Martelli MF, Grignani F, Fagioli M, Minelli O, Ciani C, et al. Expression of lymphoid-associated antigens on Hodgkin's and Reed-Sternberg cells of Hodgkin's disease. An immunocytochemical study on lymph node cytospins using monoclonal antibodies. Histopathology. 1987 Dec;11(12):1229-42. 2. Stein H, Spiegel H, Herbst H, Niedobitek G, Foss HD. [Lymphoid tissues and AIDS: role of lymphocytes and follicular dendritic cells(FDC)]Verh Dtsch Ges Pathol. 1991;75:4-19. Review. German. 3. Spiegel H, Herbst H, Niedobitek G, Foss HD, Stein H. Follicular dendritic cells are a major reservoir for human immunodeficiency virus type 1 in lymphoid tissues facilitating infection of CD4+ T-helper cells. Am J Pathol. 1992 Jan;140(1):15-22. 4. Daum S, Weiss D, Hummel M, Ullrich R, Heise W, Stein H, Riecken EO, Foss HD; Intestinal Lymphoma Study Group. Frequency of clonal intraepithelial T lymphocyte proliferations in enteropathy-type intestinal T cell lymphoma, coeliac disease, and refractory sprue. Gut. 2001 Dec;49(6):804-12.
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Tips
*Gentaur's antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals. ** The preservative sodium azide is known to be poisonous and potentially hazardous to health. It should be handled only by trained staff. Despite of the product's low azide concentration it must be handled with care. Dispose according to regional rules!
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use. CD4+ T helper cell marker or cluster differentiator of white blood cells in FACS or flow cytometry. Used offer coupled to magnetic beads. GENTAUR makes custom magnetic bead couplings on 50 and 250 nm beads for certain clones.
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Test
bio logo supplies antibodies that are for research of human proteins. Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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About
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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Latin name
Mus musculus
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Gene target
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Gene symbol
CD4
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Short name
Research
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Technique
Mouse monoclonal, anti-Human, anti-, Mouse, anti, antibody to, antibodies, antibodies against human proteins, Monoclonals or monoclonal antibodies, mouses, mouse monoclonals
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Host
mouse monoclonal, Murine monoclonal antibodies or Mabs are often conjugated and the isotype is IgG.
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Isotype
IgG2a
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Species
Human, Humans, Mouses
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Alternative name
CD4, human T helper cells
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Alternative technique
murine, antibodies
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Alternative to gene target
CD4 molecule, CD4mut, CD4 and IDBG-15034 and ENSG00000010610 and 920, protein homodimerization activity, Cell surfaces, Cd4 and IDBG-188351 and ENSMUSG00000023274 and 12504, CD4 and IDBG-640247 and ENSBTAG00000003255 and 407098
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Tissue
cells
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