CD14 FITC, Single-Color Reagent
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Catalog number
0142
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Price
Please ask
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Size
100Tests
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Host Source
Mouse
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Clone Name
FWKW
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Product Category
Flow Cytometry
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Species Reactivity
Human
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Application
Flow Cytometry
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Immunogen
NA
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Purification Method
Protein A/G Chromatography
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Shipping Conditions
Room Temperature
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Storage conditions
CD14 FITC, Single-Color Reagent should be stored at 4-8ÂșC. DO NOT FREEZE
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Properties
This Exalpha Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Exalpha FITC is highly sensitive to pH extremes.
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Conjugation
Anti-FITC Antibody
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Gene target
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Gene symbol
CD14
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Short name
CD14 FITC, Single-Color Reagent
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Technique
FITC, Fluorescein
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Isotype
IgG2b
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Label
FITC
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Alternative name
CD14 molecule fluorecein, Single-Color Reagent
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Alternative technique
fluorescine
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Alternative to gene target
CD14 molecule, CD14 and IDBG-49157 and ENSG00000170458 and 929, lipoteichoic acid binding, Extracellular, Cd14 and IDBG-138525 and ENSMUSG00000051439 and 12475, CD14 and IDBG-646064 and ENSBTAG00000015032 and 281048
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Gene info
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Identity
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Gene
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Long gene name
CD14 molecule
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Synonyms gene name
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Locus
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Discovery year
1988-05-31
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- CD molecules
- Scavenger receptors
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VEGA ID
MeSH Data
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Name
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Concept
Scope note:
Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
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Tree numbers
- E01.370.225.500.607.512.240
- E01.370.225.750.551.512.240
- E05.200.500.607.512.240
- E05.200.750.551.512.240
- E05.478.583.375
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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