-
Description
Biotin conjugated Annexin V for detection of apoptosis through fluorescence microscopy or by flow cytometry. Reagent for detecting early stages of apoptosis. During apoptosis, phosphatidylserine (PS) is translocated from the cytoplasmic face of the plasma membrane to the cell surface. Annexin V has a strong, Ca²ᶧ dependent affinity for PS and therefore is used as a probe for detecting apoptosis. The biotin-conjugated Annexin V reagent can be visualized after binding to avidin-FITC or other tags.
-
Product Highlights
•Sample- Cell culture •Formulation- 0.5 mg/ml of Annexin V-Biotin in PBS containing 1% BSA and 0.02% thimerosal. •Purity- >95% by SDS-PAGE •Simple, easy to use and convenient
-
Storage Temp
+4°C
-
Shipping
gel pack
-
Shelf Life
12 months
-
-
Notes
For research use only. Not to be used for human or animal treatment or consumption.
-
Additional description
This Annexin V calcium dependent phospholipid binding protein will be downstream of BCL2 pathway and at caspase apoptotic level. Biovision has more cell death kits and antibodies.
-
Properties
Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
-
Conjugation
Biotinylated
