MOUSE Anti-BOVINE CD80:FITC Antibody
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Catalog number
GEN224654
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Price
Please ask
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Size
100ug
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Also known as
CD80
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Other names
T-lymphocyte activation antigen CD80; CD80 anitgen; T-lymphocyte activation antigen CD80; CD80 antigen; CD80 molecule
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Category
Antibodies
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Subcategory
Mnoclonal antibodies
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Gene name
CD80
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Gene name synonims
N/A
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Other gene names
CD80; CD80; N/A; N/A
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Clonality
Monoclonal
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Immunoglobulin isotype
IgG1
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Clone
IL-A159
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Host organism
Mouse (Mus musculus)
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Species reactivity
Bovine; Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.
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Specificity and cross reactivity
CD80 Clone IL-A159 recognises the bovine CD80 cell surface antigen, which is expressed by dendritic cells, activated macrophages and activated B cells. CD80 plays a key role in co-stimulation of T cells in the primary immune response.; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.
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Purification method
N/A
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Form Appearance
FITC (Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid)
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Concentration
IgG concentration 0.1 miligram per 1 mililiter
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Storage and shipping
Store the antibody at +4 degrees Celsius for short-term storage and at -20 degrees Celsius for long-term.Storage in frost-free freezers is not recommended. the antibody should be stored undiluted. the antibody is photosensitive and should be protected from light. Repeated freeze - thaw cycles may denature the peptide chains of the antibody and therefore should be maximally avoided. If there is a precipitate in the vial we recommend you to briefly microcentrifugate it prior to use. Shelf Life: 18 months from date of dispatch.
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Tested applications
Flow Cytometry (FACS)
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Properties
If you buy Antibodies supplied by MBS Monoclonals they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This MBS Monoclonals Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MBS Monoclonals FITC is highly sensitive to pH extremes.
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Conjugation
Anti-FITC Antibody
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin name
Mus musculus
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French translation
anticorps
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Gene target
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Gene symbol
CD80
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Short name
MOUSE Anti- CD80:FITC Antibody
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Technique
Antibody, anti-, Mouse, anti, FITC, antibody to, antibodies, antibodies against human proteins, antibodies for, Fluorescein, mouses
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Host
mouse
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Label
FITC
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Species
Bovine, Bos Taurus genes are available form the Bovine Genome database. Mouses
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Alternative name
Anti-MOUSE ANTI BOVINE CD80:FITC
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Alternative technique
antibodies, murine, fluorescine
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Alternative to gene target
CD80 molecule, B7 and B7-1 and B7.1 and BB1 and CD28LG and CD28LG1 and LAB7, CD80 and IDBG-51287 and ENSG00000121594 and 941, coreceptor activity, Cell surfaces, Cd80 and IDBG-160288 and ENSMUSG00000075122 and 12519, BT.27986 and IDBG-632750 and ENSBTAG00000018059 and 407131
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Gene info
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Identity
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Gene
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Long gene name
CD80 molecule
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Synonyms gene
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Synonyms gene name
- CD80 antigen (CD28 antigen ligand 1, B7-1 antigen)
- CD80 molecule
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Synonyms
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Synonyms name
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Locus
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Discovery year
1993-12-14
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- B7 family
- CD molecules
- C2-set domain containing
- V-set domain containing
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VEGA ID
MeSH Data
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Name
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Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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