Goat, F(ab)'2 fragment, Biotin conjugated

• Catalog number
  AS10 745
• Price
  Please ask
• Size
  0.5 mg

• Available ordering format
  Liquid
• Immunogen
  purified human IgA heavy (alpha chain)
• Raised in
  Goat
• Clonality
  Polyclonal
• Clone
  Polyclonal
• Purification
  Affinity purified goat IgG
• How to reconstitute
  See included datasheet or contact our support service
• Storage condition
  Store undiluted liquid at 2-8°C. Shelf life is 1 year from date of receipt.
• Verified applications
  suitable for all immunoassays applications
• Connected products
  no related to other products
• Recommended dilutions for use
  optimal working dilution should be determined by the investigator
• Molecular weight expected аpparent
  see datasheet
• Verified reactivity
  human IgA heavy (alpha chain) F(ab)'2 fragments
• Possible reactivity
  human IgA heavy (alpha chain) F(ab)'2 fragments
• No reactivity
  no confirmed exceptions from predicted reactivity known in the moment
• Supplementary information
  No reactivity is observed to other immunoglobulins or non-immunoglobulin human serum proteins based on immunoelectrophoresis.
• References
  to be added when available
• Scientific context
  Goat anti-human IgA (alpha chain) is a secondary antibody conjugated to biotin which binds to human IgA heavy (alpha chains) F(ab)'2 fragments in immunological assays.
• Notes
  biotin conjugate is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. Contains 0.05 % (w/v) sodium azide as preservative.Purity is ≥ 90% based on SDS-PAGE. May contain small amounts of intact IgG.
• Protein number
  Refer to NCBI
• TAIR number
  Refer to NCBI
• Hyperlink
• Properties
  Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
• Conjugation
  Biotinylated
• Latin name
  Capra aegagrus hircus

• Gene target
  conjugated  
• Short name
  Goat, F(ab)'2 , conjugated
• Technique
  Goat, antibody Conjugates, goats
• Host
  Goat, The goat polyclonal antibodies are directed against human genes or rabbit or mouse IgGs (H +L) chain primary antibodies and conjugated for detection. It are very good secondary antibodies that are affinity purified and have a good affinity themselves too. Often KLH is coupled to the antigen so anti KLH can be present in the vial.
• Isotype
  Antibody fragments and scFv fragments, single chain variable fragments can be E. coli expressed. Fragment peptides for polyclonals give monoclonal like antibodies as the epitope is very small. IgG Fc and(ab)2 fragments are small and stable subunits of the antibody obtained by enzymatic digestion. Complement fragments, bp DNA fragments are common.
• Label
  Biotin
• Species
  Goats
• Alternative name
  caprine, F(Antibody)'2 fragment, biotinilated coupled
• Alternative technique
  caprine

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