IgG F(c), Human (Biotin)
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Catalog numberMBS655053
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PricePlease ask
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Size1 mg
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Products_typeImmunoglobulin
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Products_short_name[IgG F(c)]
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ReactivityHuman
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SpecificityIEP Assay shows a single precipitin arc against anti-biotin, anti-Human IgG, anti-Human IgG F(c) and anti-Human Serum. No reaction was observed against anti-Human IgG F(cab')2 or anti-Papsain.
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PurityHighly Purified Purified by ion exchange chromatography.
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FormSupplied as a lyophilized powder in PBS, pH 7.2, 10mg/ml BSA, 0.1% sodium azide. Labeled with Biotin. Biotin/Protein Ratio: 10-20 BAC molecules per Human IgG F(c) molecule. Reconstitute with 1ml sterile, distilled, deionized water or sterile 40-50% glycer
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Storage_stabilityLyophilized powder may be stored at 4 degree C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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Tested_applicationELISA (EL/EIA), Immunoprecipitation (IP), Immunohistochemistry (IHC)
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PropertiesBiotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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ConjugationBiotinylated
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AboutImmunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.
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Gene target-
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Gene symbolARSC2, CLEC4F, POTEF
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Short nameIgG F(c), ( )
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TechniqueIgG, IgGs
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HostHuman plasma
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IsotypeIgG, IgG
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LabelBiotin
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SpeciesHuman, Humans
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Alternative nameImmunoglobulin G F(c), H. sapiens (biotinilated)
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Alternative techniqueimmunoglobulins
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Gene info
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Identity
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Gene
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Long gene namearylsulfatase C, isozyme F
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Synonyms gene
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Locus
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Discovery year1988-08-31
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Entrez gene record
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Pubmed identfication
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Classification
- Sulfatases
Gene info
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Identity
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Gene
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Long gene nameC-type lectin domain family 4 member F
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Synonyms gene
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Synonyms gene name
- C-type (calcium dependent, carbohydrate-recognition domain) lectin, superfamily member 13
- C-type lectin domain family 4, member F
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Synonyms
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GenBank acession
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Locus
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Discovery year2004-02-16
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- C-type lectin domain containing
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namePOTE ankyrin domain family member F
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Synonyms gene
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Synonyms gene name
- ANKRD26-like family C, member 1B
- POTE ankyrin domain family, member F
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Synonyms
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GenBank acession
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Locus
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Discovery year2007-11-27
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- POTE ankyrin domain containing
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VEGA ID
