HAMSTER ANTI MOUSE CD137:FITC Antibody
-
Catalog number
GEN219735
-
Price
Please ask
-
Size
100ug
-
-
Also known as
CD137
-
Other names
tumor necrosis factor receptor superfamily member 9 isoform 1; Tumor necrosis factor receptor superfamily member 9; tumor necrosis factor receptor superfamily member 9; CD137 antigen; T-cell antigen 4-1BB; 4-1BB ligand receptor; secreted CD137 antigen; tumor necrosis factor receptor superfamily, member 9; 4-1BB ligand receptor; T-cell antigen 4-1BB
-
Category
Antibodies
-
Subcategory
Mnoclonal antibodies
-
Gene name
CD137
-
Gene name synonims
N/A
-
Other gene names
Tnfrsf9; Tnfrsf9; ILA; Ly63; 4-1BB; Cd137; CDw137; AA408498; AI325004; A930040I11Rik; Cd137; Ila; Ly63
-
Clonality
Monoclonal
-
Immunoglobulin isotype
IgG
-
Clone
17B5
-
Host organism
Hamster
-
Species reactivity
Mouse (Mus musculus); Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.
-
Specificity and cross reactivity
CD137 This item recognises mouse CD137, also known as 4-1BB ligand receptor, a member of the tumour necrosis factor superfamily. CD137 is a potent costimulatory receptor for the growth and functional maturation of activated T-cells in the presence of T-cell receptor signal. It is found on activated T-cells, NK cells, monocytes and dendritic cells. Signalling through CD137 triggers T-cell expansion, cytokine production and prevention of activation-induced death of effector T-cells. CD137-mediated activation is distinct from and independent of CD28. Antibody cross-linking of CD137 on NK cells stimulates the secretion of IFN-gamma, cellular proliferation, CD25 expression and responsiveness to IL-2 and is associated with tumour activity, but not cytotoxicity. _x000D__x000D_Clone 17B5 may be used to block CD137-mediated T-cell proliferation.; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.
-
Purification method
N/A
-
Form Appearance
FITC (Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid)
-
Concentration
IgG concentration 0.1 miligram per 1 mililiter
-
Storage and shipping
Store the antibody at +4 degrees Celsius for short-term storage and at -20 degrees Celsius for long-term.Storage in frost-free freezers is not recommended. the antibody should be stored undiluted. the antibody is photosensitive and should be protected from light. Repeated freeze - thaw cycles may denature the peptide chains of the antibody and therefore should be maximally avoided. If there is a precipitate in the vial we recommend you to briefly microcentrifugate it prior to use. Shelf Life: 18 months from date of dispatch.
-
Tested applications
Flow Cytometry (FACS)
-
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. This is against a protein of mus musculus directed antibody.
-
Properties
If you buy Antibodies supplied by MBS Monoclonals they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This MBS Monoclonals Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MBS Monoclonals FITC is highly sensitive to pH extremes. Armenian of Syrian hamster monoclonal IgGs are useful antibodies for double staining your IHC samples. Using mabs or monoclonal antibodies has a lot of advantages for Flow cytometry.
-
Conjugation
Anti-FITC Antibody
-
Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
-
Latin name
Mus musculus
-
French translation
anticorps
-
Gene target
-
Gene symbol
TNFRSF9
-
Short name
ANTI MOUSE CD137:FITC Antibody
-
Technique
anti mouse, Antibody, Mouse, anti, FITC, antibody to, anti mouse antibodies, antibodies against human proteins, antibodies for, Fluorescein, mouses
-
Host
Hamster
-
Label
FITC
-
Species
Mouse, Hamsters, Mouses
-
Alternative name
Anti-HAMSTER ANTI MOUSE CD137:FITC
-
Alternative technique
antibodies, murine, fluorescine
-
Gene info
MeSH Data
-
Name
-
Concept
Scope note:
A method for the study of certain organic compounds within cells, in situ, by measuring the light intensities of the selectively stained areas of cytoplasm. The compounds studied and their locations in the cells are made to fluoresce and are observed under a microscope.
-
Tree numbers
- E01.370.225.500.386
- E05.196.712.516.600.240
- E05.200.500.386
- E05.242.386
-
Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
Similar products