Adenylate Kinase (AK) Activity Assay Kit (Colorimetric/Fluorometric)

• Catalog number
  K350-100
• Price
  Please ask
• Size
  100 assays

• Description
  The most sensitive kit on the market
• Summary
  • Detection method- Absorbance (OD 570 nm) or Fluorescence (Ex/Em = 535/587 nm) • Application- Detection of Adenylate Kinase activity
• Detection Method
  Absorbance (OD 570 nm) or Fluorescence (Ex/Em = 535/587 nm)
• Applications
  Detection of Adenylate Kinase activity
• Sample Type
  • Purified Adenylate Kinase • Cell and tissue lysate • Mitochondrial lysate
• Features Benefits
  • Simple, rapid & convenient • The kit includes all the reagents required to measure AK activity
• Storage Conditions
  -20°C
• Shipping Conditions
  gel pack
• Shelf life
  12 months
• Background
  Adenylate Kinase (AK) (EC 2.7.4.3) is an abundant enzyme involved in energy metabolism and homeostasis of cellular adenine nucleotide ratios in different intracellular compartments. The enzyme is found in the nucleus, cytosol, or mitochondria (intermembrane space or matrix) of various kinds of tissues. Adenylate kinase acts on two molecules of ADP to generate ATP and AMP. Nine isoforms of adenylate kinase have been identified. Erythrocyte adenylate kinase deficiency is associated with hemolytic anemia. Adenylate kinase also plays an important role in post-ischemic recovery and in apoptosis. BioVision’s AK Activity Assay kit can kinetically measure Adenylate Kinase activity by detecting adenosine triphosphate (ATP) generated from adenosine diphosphate (ADP) as a substrate. ATP is detected via a multi-step reaction, resulting in the generation of an intermediate that reacts with the Adenylate Kinase Probe forming an end product that can be measured colorimetrically (OD 570 nm) or fluorometrically (Ex/Em = 535/587 nm).
• Data Sheet Link
• Test
  Biovision supplies other types of Assays as 1.
• Properties
  Colorimetric assays or detection use UV absorption or enzymatic color reaction.

• Gene target
  Adenylate   Kinase   Activity   Kit   Fluorometric  
• Short name
  Adenylate Kinase (AK) Activity Assay Kit (Colorimetric/Fluorometric)
• Technique
  colorimetric, Assay, assays, colorimetry
• Host
  Assay
• Alternative name
  Adenylate phosphorylation catalyst (AK) Activity test reagent (colorimetry/fluorescence)
• Alternative technique
  assays, arrays, kits
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832

Product images

Similar products