Rabbit Monoclonal IgG Anti-Mammaglobin (31A5) [31A5]

  • Catalog number
    MAM002-G
  • Price
    Please ask
  • Size
    5 ml
  • Availability
    Please contact us to check the availability
  • Immunogen
    Recombinant Mammaglobin A, whole sequence (93 aa)
  • Specificity
    10 KDa Mammaglobin from human breast tissue
  • Species reactivity
    Human, Monkey, Mouse, others not tested
  • Product presentation
    Antibody solution in stabilizing phosphate buffer pH 7.3. Contains 0.09 % sodium azide**. The volume is sufficient for at least 50 immunohistochemical tests (100 µl working solution / test). Use appropriate antibody diluent e.g. Gentaur Art. No. PU002, if further dilution is required.
  • Product category
    Primary Antibody
  • Antibody s raised in
    Rabbit
  • Antibody s isotype
    IgG
  • Antibody s clone
    31A5
  • Product is supplied as
    ready to use
  • Recommended concentration for use
    neat
  • Protein concentration
    N/A
  • Positive control
    Mamma carcinoma (normal mamma shows up lower expression of the protein), skin sweat glands
  • Pretreatment
    Formalin-fixed tissue may be pre-treated for 30 min at 100°C with Unmasking Solution C (Citrate buffer Art. No. DE000), E (buffered EDTA Art. No. DE006) or G (Glycol based solution Art. No. DE007) preferably in a pressure cooker.
  • Tested applications
    IHC(P)
  • Recommended secondary reagent
    Gentaur Universal Staining Kits Art.-No. DA005 (AE005) or VECTASTAIN Elite ABC Rabbit IgG (Art.-No. PK-6101) or VECTOR ImmPRESS™ Rabbit IgG Kit (Art. No. MP-7401)
  • Storage method
    Store at 2-8°C
  • UniProt number
    Go to UniProt/SwissProt
  • Litterature
    1. Watson MA, Dintzis S, Darrow CM, Voss LE, DiPersio J, Jensen R, Fleming TP. Mammaglobin expression in primary, metastatic, and occult breast cancer. Cancer Res. 1999 Jul 1;59(13):3028-31. 2. Fanger GR, Houghton RL, Retter MW, Hendrickson RC, Babcook J, Dillon DC,Durham MD, Reynolds LD, Johnson JC, Carter D, Fleming TP, Roche PC, Persing DH, Reed SG. Detection of mammaglobin in the sera of patients with breast cancer. Tumour Biol. 2002 Jul-Aug;23(4):212-21. 3. Han JH, Kang Y, Shin HC, Kim HS, Kang YM, Kim YB, Oh SY. Mammaglobin expression in lymph nodes is an important marker of metastatic breast carcinoma. Arch Pathol Lab Med. 2003 Oct;127(10):1330-4. 4. Gillanders WE, Mikhitarian K, Hebert R, et al. Molecular detection of micrometastatic breast cancer in histopathology-negative axillary lymph nodes correlates with traditional predictors of prognosis: an interim analysis of a prospective multi-institutional cohort study. Ann Surg. 2004 Jun;239(6):828-37; discussion 837-40.
  • Tips
    *Gentaur's antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals. ** The preservative sodium azide is known to be poisonous and potentially hazardous to health. It should be handled only by trained staff. Despite of the product's low azide concentration it must be handled with care. Dispose according to regional rules!
  • Description
    This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
  • About
    Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures. Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes. Rabbits are used for polyclonal antibody production by bio logo. Rabbit antibodies are very stable and can be stored for several days at room temperature. bio logo adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
  • Latin name
    Oryctolagus cuniculus
  • Gene target
  • Short name
    Bioprime
  • Technique
    Rabbit, anti-, anti, IgG, antibody to, antibodies, IgGs, Monoclonals or monoclonal antibodies
  • Host
    Rabbit, Rabbits
  • Isotype
    IgG, IgG
  • Alternative name
    Mammaglobin (31A5)
  • Alternative technique
    rabbit-anti, antibodies, immunoglobulins
MeSH Data
  • Name
  • Concept
    Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
  • Tree numbers
    • E05.196.401.143
    • E05.301.300.096
    • E05.478.566.320.200
    • E05.601.262
    • E05.601.470.320.200
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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