Human Recombinant PP2C alpha

• Catalog number
  6303-100
• Price
  Please ask
• Size
  100 ug

• Synonyms
  Human Recombinant PP2C alpha
• Alternative_names
  Protein phosphatase 1A (formerly 2C), human recombinant PP2C alpha, hrPP2C alpha, human PP2C alpha, recombinant PP2C alpha, magnesium-dependent, alpha isoform, PPM1A, PP2CA, PP2C-ALPHA.
• Description
  Negative regulator of cell stress response pathways
• Recombinant
  Yes
• Source
  E. coli
• Purity by SDS PAGE
  ≥95%
• Assay
  SDS-PAGE
• Endotoxin Level
  < 1.0 EU per 1 ug of protein (determined by LAL method)
• Biological activity
  >8,000 units/mg.
• Unit Definition
  Enzymatic activity is defined as the amount of enzyme hydrolyzing 1 nmole of p-nitrophenyl phosphate (pNPP) per minute at 37°C, pH 7.5 using 10 mM of substrate.
• Molecular Weight
  46.6 kDa (418aa)
• Storage Temp
  -20°C
• Shipping
  gel pack
• Shelf Life
  12 months
• Concentration
  1 mg/ml
• Appearance
  Liquid
• Physical form description
  1 mg/ml solution in 10 mM Tris-HCl, pH 7.5, 50 mM NaCl, 2 mM DTT, 1 mM MnCl2, 20% glycerol.
• Background Information
  Protein phosphatase 2C (PP2C alpha) is a Mn2+- or Mg2+- dependent protein serine/threonine phosphatase that is essential for regulating cellular stress response in eukaryotes. The protein coding region of PP2C alpha (amino acids 1-382) was cloned into an E. coli expression vector. PP2C alpha was overexpressed in E. coli as a soluble His-tag fusion protein, and it was purified by conventional column chromatographic techniques.
• Amino acid sequence
  MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWIL FFAVYDGHAG SQVAKYCCEH LLDHITNNQD FKGSAGAPSV ENVKNGIRTG FLEIDEHMRV MSEKKHGADR SGSTAVGVLI SPQHTYFINC MGAF LDKPKMEKHN AQGQGNGLRY GLSSMQGWRV EMEDAHTAVI GLPSGLESWS GDSRGLLCRN RKVHFFTQDH KPSNPLEKER IQNAGGSVMI QRVNGSLAVS RALGDFDYKC VHGKGPTEQL VSPEPEVHDI ERSEEDDQFI ILACDGIWDV MGNEELCDFV RSRLEVTDDL EKVCNEVVDT CLYKGSRDNM SVILICFPNA PKVSPEAVKK EAELDKYLEC RVEEIIKKQG EGVPDLVHVM RTLASENIPS LPPGGELASK RNVIEAVYNR LNPYKNDDTD STSTDDMW
• Handling
  Centrifuge the vial prior to opening.
• Usage
  For Research Use Only! Not to be used in humans
• Datasheet
• Additional description
  The Recombinant PP2C alpha is a α- or alpha protein sometimes glycoprotein present in blood.
• Properties
  Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Additional source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  PP2C   alpha  
• Gene symbol
  PPTC7, PPM1D
• Short name
  Recombinant PP2C alpha
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
• Species
  Human, Humans
• Alternative name
  H. sapiens Rec. PP2C a
• Alternative technique
  rec

Gene info

• Identity
  HGNC:30695
• Gene
  PPTC7
• Long gene name
  protein phosphatase targeting COQ7
• Synonyms gene name
  • PTC7 protein phosphatase homolog (S. cerevisiae)
• Synonyms
  • TA-PP2C
• Synonyms name
  • T cell activation protein phosphatase 2C
• GenBank acession
  • AF385435
• Locus
  12q24.11
• Discovery year
  2006-02-27
• Entrez gene record
  160760
• Pubmed identfication
  • 15177553
  • 30267671
• RefSeq identity
  • NM_139283
• Classification
  • Protein phosphatases, Mg2+/Mn2+ dependent
• VEGA ID
  OTTHUMG00000169529

Gene info

• Identity
  HGNC:9277
• Gene
  PPM1D
• Long gene name
  protein phosphatase, Mg2+/Mn2+ dependent 1D
• Synonyms gene name
  • protein phosphatase 1D magnesium-dependent, delta isoform
  • protein phosphatase, Mg2+/Mn2+ dependent, 1D
• Synonyms
  • Wip1
  • PP2C-DELTA
• Synonyms name
  • wild-type p53-induced phosphatase 1
  • protein phosphatase 2C, delta isoform
• GenBank acession
  • U78305
• Locus
  17q23.3
• Discovery year
  1998-10-14
• Entrez gene record
  8493
• Pubmed identfication
  • 9177166
• RefSeq identity
  • NM_003620
• Classification
  • Protein phosphatases, Mg2+/Mn2+ dependent
• VEGA ID
  OTTHUMG00000180052
• Locus Specific Databases
  • LRG_770

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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