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Synonyms
Recombinant CFP Protein, Cyan Fluoroscent Protein
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Alternative_names
CFP, Cyan Fluorescent Protein
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Description
Fluorescent protein ideal for subcellular labeling/visualization
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Recombinant
Yes
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Source
E. coli
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Purity by SDS PAGE
≥97%
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Assay
SDS-PAGE
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Purity
≥97%
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Molecular Weight
31.3 kDa
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Storage Temp
-20°C
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Shipping
Gel pack
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Shelf Life
12 months
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Appearance
Lyophilized protein
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Physical form description
Freeze Dried
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Reconstitution Instructions
Reconstitute with dH₂O to 1 mg/ml
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Background Information
The recombinant CFP (Cyan Fluorescent Protein) is expressed and purified from transformed E. coli using a method that ensures high purity and maximal CFP fluorescence. Endotoxin has been removed, so the protein is suitable for in vivo injection or cell culture applications. The protein is a 31.3 kDa monomer with 284 amino acids and an N-terminal His-tag, Ex./Em. = 458/480 nm, extinction coefficient 26000M-1CM-1.
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Handling
Centrifuge the vial prior to opening.
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Usage
For Research Use Only! Not to be used in humans
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Additional description
Fluorescent microspheres, beads and particles applications including blood flow determination, tracing, fluorimetry, in vivo imaging and calibration of imaging and flow cytometry instruments. Because our fluorescent dyes are incorporated in the bead and not just on the surface, they are relatively immune to photo bleaching and other environmental factors. Spheres are in difference sizes and fluorescences, high intensity, FITC, GFP, red, green, yellow, light yellow, sky blue, blue, orange, deep-red, Nile-red, purple, mcherry. The diameter of the spheres is usually higher than 50nm and in the micrometer um range.
