BMI 1 Antibody

  • Catalog number
    6150-100
  • Price
    Please ask
  • Size
    100 μg
  • Antibody Target
    BMI 1
  • Target Alternative Name
    RNF51, PCGF4
  • Antibody Type
    Polyclonal
  • Species Reactivities
    Human, mouse, rat
  • Immunogen Sequence
    Recombinant protein of human BMI1
  • Accession
    P35226
  • Gene Id
    648
  • Appearance
    Colorless liquid
  • Form
    Liquid
  • Concentration
    1 mg/ml
  • Formulation
    100 µg of antibody in 100 µl PBS containing 0.02% sodium azide, 50% glycerol, pH 7.3
  • Purification
    Affinity purified
  • Application
    Western blot, Immunohistochemistry
  • Positive Control
    Western Blot: K562 cell lysates
  • Application And Usages
    Western blot: 1:500 – 1:2000, IHC: 1:50 – 1:200.
  • Description
    The polycomb group (PcG) of proteins contributes to the maintenance of cell identity, stem cell self-­renewal, cell cycle regulation, and oncogenesis by maintaining the silenced state of genes that promote cell lineage specification, cell death, and cell cycle arrest. PcG proteins exist in two complexes that cooperate to maintain long term gene silencing through epigenetic chromatin modifications. The first complex, EED­EZH2, is recruited to genes by DNA­binding transcription factors and methylates histone H3 on Lys27. This histone methyl­transferase activity requires the Ezh2, Eed, and Suz12 subunits of the complex. Histone H3 methylation at Lys27 facilitates the recruitment of the second complex, PRC1, which ubiquitinylates histone H2A on Lys119. Bmi1 is a component of the PRC1 complex, which together with Ring1 strongly enhances the E3 ubiquitin ligase activity of the Ring2 catalytic subunit. Bmi1 plays an important role in the regulation of cell proliferation and senescence through repression of the p16 INK4A and p19 ARF genes and is required for maintenance of adult hematopoietic and neural stem cells.
  • Storage Condition
    -20 °C
  • Shipping Condition
    gel pack
  • Shelf Life
    12 months
  • Usage
    For Research Use Only! Not to be used in humans.
  • Handling
    The antibody solution should be gently mixed before use.
  • Western Blot Verified
    Verified
  • Immunocytochemistry Verified
    Not Verified
  • Immunofluorescence Verified
    Not Verified
  • Immunoprecipitation Verified
    Not Verified
  • ELISA Verified
    Not Verified
  • ChIP Verified
    Not Verified
  • Flow Cytometry Verified
    Not Verified
  • Properties
    If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
  • French translation
    anticorps
  • Gene target
    BMI  
  • Short name
    BMI 1 Antibody
  • Technique
    Antibody, antibodies against human proteins, antibodies for
  • Isotype
    Rabbit IgG
  • Alternative name
    BMI 1 (antibody to-)
  • Alternative technique
    antibodies
MeSH Data
  • Name
  • Concept
    Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
  • Tree numbers
    • E05.196.401.143
    • E05.301.300.096
    • E05.478.566.320.200
    • E05.601.262
    • E05.601.470.320.200
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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