Mouse anti 6x-His Tag

  • Catalog number
    X1181M
  • Price
    Please ask
  • Size
    100 μg
  • Category
    Primary Antibodies
  • Long description
    6x-His tags are a type of tag for expressed proteins. This tag is made up of 6 histidine residues attached to either the N- or C-terminal of a protein. Proteins expressed with this type of tag are then traditionally purified using a divalent metal ion column. However, using an antibody specific to this 6x-His tag, the protein can be analyzed for by Western blot or immunofluorescence techniques, thus eliminating the need for a protein-specific antibody. 6x-His tags are particularly effective for the purification of protein expressed in E. coli systems. This antibody has been found to be effective in detecting proteins expressed using the following vector systems, pET 28, 30 and 32 as well as Invitrogen's pIZ and is specific for 6x-His tags only.
  • Antibody come from
    Hybridoma produced by the fusion of splenocytes from mice immunized with His-His-His-His-His-His synthetic peptide and mouse myeloma cells.
  • Other description
    Provided as solution in phosphate buffered saline with 0.08% sodium azide. Protein A/G Chromatography
  • Clone
    2C5.1
  • Antigen antibody binding interaction
    Mouse anti 6x-His Tag Antibody
  • Antibody is raised in
    Mouse
  • Antibody s reacts with
    N/A
  • Antibody s reacts with these species
    This antibody doesn't cross react with other species
  • Antibody s specificity
    No Data Available
  • Application
    Western Blot
  • Antibody s suited for
    Detects only 6x-His tag by Western blot. Specific for His tags no longer than 6 histidines. Optimal concentration should be evaluated by serial dilutions.
  • Storage
    -20ºC
  • Relevant references
    1. Zentgraf, H., et al. Detection of histidine-tagged fusion proteins by using a high-specific mouse monoclonal anti-histidine tag antibody. Nucleic Acids Res. 1995, 23, 3347-3348_x000B__x000B_2. Sisk, W.P., et al. High-level expression and purification of secreted forms of herpes simplex virus type 1 glycoprotein gD synthesized by baculovirus-infected insect cells. J. Virol. 1994, 68, 766-775_x000B__x000B_3. Hosfield, T. and Lu, Q. S. pombe expression vector with 6x(His) tag for protein purification and potential for ligation-independent cloning. Biotechniques 1999, 27, 50-60
  • Protein number
    see ncbi
  • Warnings
    This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
  • Description
    This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
  • Conjugation
    histidine
  • Test
    Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
  • Latin name
    Mus musculus
  • Gene target
    6x-His   Tag  
  • Short name
    Mouse anti 6x-His Tag
  • Technique
    Mouse, anti, antibody to, mouses, Tags
  • Host
    mouse
  • Isotype
    IgG2a
  • Label
    unlabeled
  • Species
    Mouse, Mouses
  • Alternative name
    Mouse antibody to 6x-histidine detection labelled
  • Alternative technique
    murine, antibodies
MeSH Data
  • Name
  • Concept
    Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
  • Tree numbers
    • E05.196.401.143
    • E05.301.300.096
    • E05.478.566.320.200
    • E05.601.262
    • E05.601.470.320.200
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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