Recombinant Rat E3 ubiquitin-protein ligase RNF5 (Rnf5)

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About

Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
Latin name

Rattus norvegicus
Source

Recombinants or rec. proteins
Group

recombinants

Gene target

ubiquitin-protein ligase RNF5 Rnf5
Gene symbol

RNF5
Short name

Recombinant E3 ubiquitin-protein ligase RNF5 (Rnf5)
Technique

Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. MyBioSource advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
Host

Rat
Species

Rat, Rats
Alternative name

Rec. Rat E3 ubiquitin-protein ligase RNF5 (Rnf5)
Alternative technique

rec

Name

Ligase Chain Reaction
Concept

Scope note: A DNA amplification technique based upon the ligation of OLIGONUCLEOTIDE PROBES. The probes are designed to exactly match two adjacent sequences of a specific target DNA. The chain reaction is repeated in three steps in the presence of excess probe: (1) heat denaturation of double-stranded DNA, (2) annealing of probes to target DNA, and (3) joining of the probes by thermostable DNA ligase. After the reaction is repeated for 20-30 cycles the production of ligated probe is measured.
Tree numbers
- E05.393.620.311
Qualifiers

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