Akt3, Active

  • Catalog number
    7703-100
  • Price
    Please ask
  • Size
    100 ug
  • Synonyms
    Akt, RAC-alpha serine/threonine-protein kinase
  • Alternative_names
    Akt, RAC-alpha serine/threonine-protein kinase
  • Description
    A serine/threonine-protein kinase
  • Recombinant
    Yes
  • Source
    Baculovirus (Sf9 insect cells)
  • Purity by SDS PAGE
    ≥90%
  • Assay
    SDS-PAGE
  • Activity Specifications test method
    206 nmol phosphate incorporated into Akt substrate peptide per minute per mg protein at 30°C for 15 minutes using a final concentration of 50 µM ATP and total of 0.83 µCi/µl P-32.
  • Molecular Weight
    ~84.0 kDa
  • Storage Temp
    -80°C
  • Shipping
    Dry ice
  • Shelf Life
    12 months
  • Concentration
    5 µg/50 µl
  • Appearance
    Liquid
  • Physical form description
    Recombinant proteins in storage buffer (50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol).
  • Background Information
    Akt3 or Protein Kinase B γ (PKB) is a serine/threonine kinase that is a member of the Akt family. In mammals, the Akt family comprises of three highly homologous members known as Akt1 (PKB), Akt2 (PKB), and Akt 3. Akt 3 like the other family members is activated in cells exposed to diverse stimuli such as hormones, growth factors, and extracellular matrix components. The activation mechanism remains to be fully characterized but occurs downstream of phosphoinositide 3-kinase (PI3K). PI3K generates phosphatidylinositol-3,4,5-trisphosphate (PIP3), a lipid second messenger essential for the translocation of Akt family members to the plasma membrane where they are phosphorylated and activated by phosphoinositide-dependent kinase-1 (PDK-1) and phosphoinositide-dependent kinase-2 (PDK-2 possibly ILK). Akt 3 like the other family members phosphorylates and regulates the function of many cellular proteins involved in processes that include cellular metabolism, survival/apoptosis, and proliferation. Recent evidence indicates that Akt 3 is frequently overexpressed in many types of human cancers including breast and prostate. Although the mechanisms have not yet been fully characterized, increased expression and activation of Akt is believed to promote cell proliferation and survival thereby contributing to cancer progression.
  • Gene target
    Akt3   Active  
  • Short name
    Akt3, Active
  • Alternative name
    Akt3, functionnal
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