anti-BHMT Antibody
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Catalog number46-634
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PricePlease ask
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Size0.1 mg
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Gene namesbetaine-homocysteine methyltransferase, BHMT, BHMT1, HEL-S-61p
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Antibodie s labelunlabelled serum
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PurityBHMT antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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Ab raised inGoat
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TypePolyclonal
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AntigenBHMT antibody was made against a 12 amino acid synthetic peptide near the C-Terminus of BHMT.
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Immuno applicationELISA, Western Blot, immuno-histo-chemistry
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Antibody adviceELISA: Antibody detection limit dilution 1:16000. Western Blot: Approximately 45 kDa band observed in human liver, mouse liver and rat liver lysates (calculated MW of 44.9 kDa according to human NP_001704.1, 45.0 kDa according to mouse NP_057877.1 and rat NP_110477.1). In transfected HEK293 transiently expressing BHMT a band of approximately 49 kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.03-0.1 ug/mL. Immunohistochemistry: In paraffin embedded human kidney shows strong cytoplasm staining in PCT cells. Recommended concentration, 2-4 ug/mL.
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Suspention pH azideBHMT antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin.
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Keep atAliquot and store at -20 grades C. Minimize freezing and thawing.
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Works withHuman, Mouse, Rat
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Omim nr4502407
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Ncbi nrNP_001704.1
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Vial with antibodyin solution
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Quantity per ml500 ug/mL
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NCBI GENE nr635
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GENE symbolBHMT
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Antigene namebetaine--homocysteine S-methyltransferase
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SourceHomo sapiens
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Protein nrQ93088
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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PropertiesIf you buy Antibodies supplied by proscience they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translationanticorps
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Gene target
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Gene symbolBHMT
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Short nameanti-BHMT Antibody
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TechniqueAntibody, anti-, anti, antibody to, antibodies, antibodies against human proteins, antibodies for
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Alternative nameAntibody toBHMT (antibody to-)
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Alternative techniqueantibodies
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Gene info
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Identity
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Gene
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Long gene namebetaine--homocysteine S-methyltransferase
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1997-06-09
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Homocysteine methyltransferases
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data