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Available ordering format
Lyophilized
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Immunogen
His-tagged full length recombinant UAGPase of Arabidopsis thaliana, overexpressed and purified from E.coli, UniProt:O64765,, TAIR:AT2G35020. Sequence used for immunization is conserved in both isoforms: UDP-N-acetylglucosamine diphosphorylase 1 (Q940S3) and (O64765)
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Raised in
Rabbit
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Clonality
Polyclonal
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Clone
Polyclonal
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Purification
Serum
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How to reconstitute
For reconstitution add 50 µl of sterile water.
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Storage condition
store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
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Verified applications
western blot (WB)
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Connected products
collection of antibodies to carbohydrate metabolismrecommended secondary antibodyPlant protein extraction buffer Secondary antibodies
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Recommended dilutions for use
1: 10 000 with ECL (WB)
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Molecular weight expected аpparent
55.76 kDa
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Verified reactivity
Arabidopsis thaliana, Hordeum vulgare, Nicotiana tabacum
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Possible reactivity
Glycine soja, Medicago truncatula, Morus notabilis, Populus trichocarpa, Theobroma cacao, Zea mays, for more species, please inquire
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No reactivity
no confirmed exceptions from predicted reactivity known in the moment
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Supplementary information
This antibody is recognizing recombinant UAGPase at 0.25 pmol.
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References
Kleczkowski LA & Decker DD (2015) Sugar activation for production of nucleotide sugars as substrates for glycosyltransferases in plants. J. Appl. Glycosci. (in press).
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Scientific context
UAGPase (UDP-GlcNAc pyrophosphorylase) is involved in the biosynthesis of UDP-glucosamine, an essential precursor for glycoprotein and glycolipid synthesis and is also used for regulatory protein modification in signaling pathways. The enzyme is localized in a cytoplasm. Alternative names: N-acetylglucosamine-1-phosphate uridylyltransferase 2, UDP-N-acetylgalactosamine diphosphorylase 2, UTP--glucose-1-phosphate uridylyltransferase 2, AGX.
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Notes
None
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Protein number
O64765
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TAIR number
AT2G35020
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