RPMI 1640 w/ L- Glutamine w/o Sodium Bicarbonate w/ 25 mM Hepes w/o Phenol Red - For 10L

  • Catalog number
    PM-R1648/10L
  • Price
    Please ask
  • Size
    5X 10L
  • Description
    RPMI 1640 w/ L- Glutamine w/o Sodium Bicarbonate w/ 25 mM Hepes w/o Phenol Red - For 10L is used in cultures in vitro, suspension and monolayer culture of human leukemic cells.
  • Shipping conditions
    -20°C or dry ice
  • Storage
    + 2 / + 8 ° C 
  • Shelflife
    24 months
  • State
    Powder
  • Chemical description
    Mixture composed of inorganic substanced
  • Appearance
    Physical state at 20 ºC: Solid ; Colour: White
  • Density at 20 ºC
    1655 kg/m³
  • Relative density at 20 ºC
    1.655
  • PH
    6.7 ± 0.3
  • Osmolality
    260 mOsm/kg ±10 %
  • Cell growth test
    NA
  • Composition
    NA
  • Sterility
    Non-sterile 
  • Personal precautions
    Wear clothes adapted to the manipulation of serum to avoid contamination (e.g. : gloves, mask, hygiene cap, overall…)
  • Uses
    Used in biotechnology. For professional user/industrial user only.
  • Ordering
    To order RPMI 1640 w/ L- Glutamine w/o Sodium Bicarbonate w/ 25 mM Hepes w/o Phenol Red - For 10L, please use the Cat. Nr.PM-R1648/10L and submit your purchase order by email or by fax. A discount is available for larger or bulk quantities, please contact us for more information
  • Tips
    Our specialists recommend you to follow carefully the pre-registered instructions for RPMI 1640 w/ L- Glutamine w/o Sodium Bicarbonate w/ 25 mM Hepes w/o Phenol Red - For 10L
  • Buffer
    25mM HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid ) pH7 solution buffers are a media with a zwitterion organic chemical buffering agent; one of the twenty Good's buffers. HEPES DMEM medium is widely used in cell culture, largely because it is better at maintaining physiological pH 7 tot 7,5 despite changes in carbon dioxide concentration (produced by cellular respiration) when compared to bicarbonate buffers, which are also commonly used in cell culture. The dissociation of water decreases with falling temperature, but the dissociation constants (pK) of many other buffers do not change much with temperature. HEPES, 25 mM in RPMI and DMEM is like water in that its dissociation decreases as the temperature decreases. This makes HEPES RPMI 25 milli-molar media with or without L-glutamine a more effective buffering agent for maintaining enzyme structure and function at low temperatures.
  • Conjugation
    red dye
  • Gene target
    RPMI   1640   Glutamine   w/o   Sodium   Bicarbonate   w/o   Phenol   Red   For   10L  
  • Short name
    RPMI 1640 w/ L- Glutamine w/o Sodium Bicarbonate w/ 25 mM w/o Phenol Red - For 10L
  • Label
    Red
  • Alternative name
    RPMI 1640 w/ L- Glutamine w/o Sodium Bicarbonate w/ 25 millimeter Hepes w/o Phenol Red - to measure 10L
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