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Available ordering format
Lyophilized in PBS pH 7.4
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Immunogen
KLH-conjugated synthetic peptide conserved across all known sequences of HSP70 P08107 and HSC70 proteins P11142
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Raised in
Rabbit
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Clonality
Polyclonal
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Clone
Polyclonal
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Purification
Affinity purified serum
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How to reconstitute
For reconstitution add 50 µl of sterile water
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Storage condition
store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
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Verified applications
Western blot (WB), immunoprecipitation (IP)
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Connected products
Secondary antibodies
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Recommended dilutions for use
1: 1000 with standard ECL (WB), 1: 10 000 with ECL Advance (GE Healthcare), 1: 1000 (IP)
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Molecular weight expected аpparent
70 kDa
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Verified reactivity
fish, mammals, fungi: Antrodia infirma, A. sinuosa, A. xantha, Gloeophyllum protractum, G. sepiarium, G. carbonarium, Oligoporus sericiomollis, Phlebia cornea, Junghunia luteoalba
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Possible reactivity
vertebrates including bovine, hen, mouse, rat; insects including Drosophila melanogaster
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No reactivity
no confirmed exceptions from predicted reactivity known in the moment
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Supplementary information
this antibody is not suitable for work with samples from higher plants
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References
French et al. (2015). High survivorship after catch-and-release fishing suggests physiological resilience in the endothermic shortfin mako shark (Isurus oxyrinchus). Conservation Physiology, Vol 3, Issue 1, 10.1093/conphys/cov044
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Scientific context
Heat shock protein 70 (Hsp70) is the major stress-inducible protein in vertebrates and is highly conserved throughout evolution. It plays a role as a molecular chaperone and is important for allowing cells to cope with acute stress or insult, especially those affecting the protein machinery. Heat shock cognate protein 70 (HSC70) is a highly conserved protein and a member of the family of molecular chaperones. Alternative names: HSP70.1, HSP70-1/HSP70-2, Heat shock 70 kDa protein 8
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Notes
for detection of plant and algal cytoplasmic hsp70 we recommend following product: AS08 371
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Protein number
P08107 , P11142
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TAIR number
Refer to NCBI
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Test
A high affinity purification column was use to purify HSP, Heat shock cognate protein 70, by agisera by chromatographic size exclusion.
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Description
1 of protein A or G purified by agisera will give more specificity than crude serum.
