DMEM / Ham's F-12 w/ L-glutamine and 15 mM HEPES w/o phenol red, L-tryptophan and sodium bicarbonate

  • Catalog number
    DFP05-50LT
  • Price
    Please ask
  • Size
    50 L
  • Availability
    Please contact us to check current availability
  • Long description
    DMEM / Ham's F-12 w/ L-glutamine and 15 mM HEPES w/o phenol red, L-tryptophan and sodium bicarbonate Medium
  • Product format
    Powder
  • Product category
    Animal Cell Culture Media
  • Product subcategory
    DMEM / Ham's F-12
  • Shipping method
    Ambient temperature
  • Storage method
    Store at 2Ëš to 8ËšC.
  • Buffer
    25mM HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid ) pH7 solution buffers are a media with a zwitterion organic chemical buffering agent; one of the twenty Good's buffers. HEPES DMEM medium is widely used in cell culture, largely because it is better at maintaining physiological pH 7 tot 7,5 despite changes in carbon dioxide concentration (produced by cellular respiration) when compared to bicarbonate buffers, which are also commonly used in cell culture. The dissociation of water decreases with falling temperature, but the dissociation constants (pK) of many other buffers do not change much with temperature. HEPES, 25 mM in RPMI and DMEM is like water in that its dissociation decreases as the temperature decreases. This makes HEPES RPMI 25 milli-molar media with or without L-glutamine a more effective buffering agent for maintaining enzyme structure and function at low temperatures.
  • Conjugation
    red dye
  • Gene target
  • Gene symbol
    F12, HLA-F, H3C11, IGHV1-69-2, SNAR-F, KLRC4, PIRC55, PIRC57, PIRC53
  • Short name
    DMEM/F-12
  • Label
    Red
  • Alternative name
    DMEM / Ham's F-12 w/ L-glutamine and 15 millimeter HEPES w/o phenol red, L-tryptophan and sodium bicarbonate
Gene info
Gene info
Gene info
Gene info
Gene info
  • Identity
  • Gene
  • Long gene name
    small NF90 (ILF3) associated RNA F
  • Synonyms gene name
    • small nuclear ILF3/NF90-associated RNA F
    • small ILF3/NF90-associated RNA F
  • GenBank acession
  • Locus
  • Discovery year
    2008-06-20
  • Entrez gene record
  • Pubmed identfication
  • RefSeq identity
  • Classification
    • Small NF90 (ILF3) associated RNAs
Gene info
  • Identity
  • Gene
  • Long gene name
    killer cell lectin like receptor C4
  • Synonyms gene name
    • killer cell lectin-like receptor subfamily C, member 4
  • Synonyms
  • GenBank acession
  • Locus
  • Discovery year
    1998-09-03
  • Entrez gene record
  • Pubmed identfication
  • RefSeq identity
  • Classification
    • Killer cell lectin like receptors
  • VEGA ID
Gene info
  • Identity
  • Gene
  • Long gene name
    piwi-interacting RNA cluster 55
  • Locus
    12
  • Discovery year
    2009-11-05
  • Entrez gene record
  • Pubmed identfication
  • Classification
    • Piwi-interacting RNA clusters
Gene info
  • Identity
  • Gene
  • Long gene name
    piwi-interacting RNA cluster 57
  • Locus
    12
  • Discovery year
    2009-11-05
  • Entrez gene record
  • Pubmed identfication
  • Classification
    • Piwi-interacting RNA clusters
Gene info
  • Identity
  • Gene
  • Long gene name
    piwi-interacting RNA cluster 53
  • Locus
    12
  • Discovery year
    2009-11-05
  • Entrez gene record
  • Pubmed identfication
  • Classification
    • Piwi-interacting RNA clusters
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