Mouse anti Cytokeratin 8 / Keratin K8

• Catalog number
  MUB0318P
• Price
  Please ask
• Size
  0,1 mg

• Category
  Primary Antibodies
• Long description
  Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in epithelial tissues by at least 20 different polypeptides. They range in molecular weight between 40 kDa and 68 kDa and isoelectric pH between 4.9 – 7.8. The individual Cytokeratin polypeptides are numbered 1 to 20. The various epithelia in the Human body usually express Cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of matuRation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The Cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays.
• Antibody come from
  M20 is a Mouse monoclonal IgG1 antibody derived by fusion of murine myeloma cells with spleen cells from a Mouse immunized with Keratin isolated from the Human breast carcinoma cell line MCF-7.
• Other description
  Each vial contains 100 ul 1 mg/ml purified monoclonal antibody in PBS containing 0.09% sodium azide.
• Clone
  M20
• Antigen antibody binding interaction
  Mouse anti Cytokeratin 8 / Keratin K8 Antibody
• Antibody is raised in
  Mouse
• Antibody s reacts with
  Human,Rabbit,Rat
• Antibody s reacts with these species
  This antibody doesn't cross react with other species
• Antibody s specificity
  M20 reacts with glandular epithelial cells (endocrine and exocrine) as well as mesothelial cells and epithelia of the digestive, respiRatory and urogenital tract. Also most adenocarcinomas derived from these cells are positive. The M20 epitope has been mapped to amino acids 353-367 on the alpha helical rod domain of Cytokeratin 8 (see reference 10).
• Research interest
  Cytoskeleton
• Application
  Flow Cytometry,Immunocytochemistry,Immunohistochemistry (frozen),Immunohistochemistry (paraffin),Western blotting
• Antibody s suited for
  M20 is suitable for immunoblotting, immunocytochemistry, immunohistochemistry on frozen and paraffin-embedded tissues and flow cytometry. Optimal antibody dilution should be determined by titration; recommended range is 1:100 – 1:200 for immunohistochemistry with avidin-biotinylated Horseradish peroxidase complex (ABC) as detection reagent, and 1:100 – 1:1000 for immunoblotting applications.
• Storage
  Store at 4°C, or in small aliquots at -20°C.
• Relevant references
  Van Muijen, G. N., Ruiter, D. J., and Warnaar, S. O. (1987). Coexpression of intermediate filament polypeptides in Human fetal and adult tissues. Lab Invest 57, 359-69. Van Muijen, G. N., Warnaar, S. O., and Ponec, M. (1987). Differentiation-related changes of Cytokeratin expression in cultured Keratinocytes and in fetal, newborn, and adult epidermis. Exp Cell Res 171, 331-45. Schaafsma, H. E., Ramaekers, F. C., van Muijen, G. N., Ooms, E. C., and Ruiter, D. J. (1989). Distribution of Cytokeratin polypeptides in epithelia of the adult Human urinary tract. Histochemistry 91, 151-9. Smedts, F., Ramaekers, F., Robben, H., Pruszczynski, M., van Muijen, G., Lane, B., Leigh, I., and Vooijs, P. (1990). Changing patterns of Keratin expression during progression of cervical intraepithelial neoplasia. Am J Pathol 136, 657-68. Ramaekers, F., van Niekerk, C., Poels, L., Schaafsma, E., Huijsmans, A., Robben, H., Schaart, G., and Vooijs, P. (1990). Use of monoclonal antibodies to Keratin 7 in the differential diagnosis of adenocarcinomas. Am J Pathol 136, 641-55. Schaafsma, H. E., Ramaekers, F. C., van Muijen, G. N., Lane, E. B., Leigh, I. M., Robben, H., Huijsmans, A., Ooms, E. C., and Ruiter, D. J. (1990). Distribution of Cytokeratin polypeptides in Human transitional cell carcinomas, with special emphasis on changing expression patterns during tumor progression. Am J Pathol 136, 329-43. Ivanyi, D., Groeneveld, E., Van Doornewaard, G., Mooi, W. J., and Hageman, P. C. (1990). Keratin subtypes in carcinomas of the uterine cervix: impliCations for histogenesis and differential diagnosis. Cancer Res 50, 5143-52. Wetzels, R. H., Kuijpers, H. J., Lane, E. B., Leigh, I. M., Troyanovsky, S. M., Holland, R., van Haelst, U. J., and Ramaekers, F. C. (1991). Basal cell-specific and hyperprolifeRation-related Keratins in Human breast cancer. Am J Pathol 138, 751-63. Ku, N. O., Gish, R., Wright, T. L., and Omary, M. B. (2001). Keratin 8 mutations in patients with cryptogenic liver disease. N Engl J Med 344, 1580-7. Waseem, A., Karsten, U., Leigh, I.M., Purkis, P., Waseem, H. and Lane, B. (2004). Conformational changes in the rod domain of Human Keratin 8 following heterotypic association with Keratin 18 and its impliCation for filament stability. Biochemistry 43, 1283-95.
• Protein number
  P05787
• Warnings
  This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This product contains sodium azide. To prevent formation of toxic vapors, do not mix with strong acidic solutions. To prevent formation of potentially explosive metallic azides in metal plumbing, always wash into drain with copious quantities of water. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
• Antibody s link
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
• Test
  Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
• Latin name
  Mus musculus

• Gene target
  Cytokeratin   Keratin  
• Short name
  Mouse anti Cytokeratin 8 / Keratin K8
• Technique
  Mouse, anti, antibody to, mouses
• Host
  mouse
• Isotype
  IgG1
• Label
  unlabeled
• Species
  Mouse, Mouses
• Alternative name
  Mouse antibody to Cytokeratin 8 / Keratin K8
• Alternative technique
  murine, antibodies

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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