LC3 (APG8) Antibody (Clone 166AT1234)

• Catalog number
  3233-100
• Price
  Please ask
• Size
  100 µl

• Antibody Target
  LC3
• Target Alternative Name
  MAP1LC3A; Microtubule-associated proteins 1A/1B light chain 3A; Autophagy-related protein LC3 A; Autophagy-related ubiquitin-like modifier LC3 A; MAP1 light chain 3-like protein 1; MAP1A/MAP1B light chain 3 A; Microtubule-associated protein 1 light chain 3 alpha
• Antibody Type
  Monoclonal
• Clone
  166AT1234
• Species Reactivities
  Human, Mouse, Rat
• Immunogen Sequence
  This antibody is generated from mouse immunized with a full length recombinant protein of human LC3 (APG8).
• Accession
  Q9H492
• Gene Id
  84557
• Appearance
  Colorless liquid
• Form
  Liquid
• Formulation
  Supplied in PBS with 0.09% (W/V) sodium azide.
• Purification
  Affinity Purified Antibody
• Application
  Western blot, Immunohistochemistry, IF
• Positive Control
  WB: Hela cell lysate, IHC: muscle tissue, IF: U251 cells
• Application And Usages
  Western blot: 1:1000, IHC: 1:50-100, IF: 1:200.
• Description
  Autophagy is an alternative process of proteasomal degradation for some long-lived proteins or organelles. Alterations in the autophagic-lysosomal compartment have been linked to neuronal death in many neurodegenerative disorders as well as in transmissible neuronal pathologies (prion diseases). Genetic studies in yeast have shown that Autophagy-defective Gene-8 (Atg-8) represents a specific marker for autophagy. Among the four families of mammalian Atg8-related proteins only LC3 (Microtubule-associated Protein1 Light Chain 3) is expressed at sufficient high levels and efficiently recruited to autophagic vesicles in cells and tissues. During autophagy the cytoplasmic form, LC3-I is processed and recruited to autophagosomes, where LC3-II is generated by site specific proteolysis near to the C-terminus. Autophagic vacuoles have been also reported frequently in cardiomyopathies or muscle cells exposed to different experimental settings.
• Storage Condition
  -20 °C
• Shipping Condition
  gel pack
• Shelf Life
  12 months
• Usage
  For Research Use Only! Not to be used in humans.
• Handling
  The antibody solution should be gently mixed before use.
• Western Blot Verified
  Verified
• Immunocytochemistry Verified
  Verified
• Immunofluorescence Verified
  Verified
• Immunoprecipitation Verified
  Not Verified
• ELISA Verified
  Not Verified
• ChIP Verified
  Not Verified
• Dot Blot Verified
  Not Verified
• Flow Cytometry Verified
  Not Verified
• Data Sheet Link
• Properties
  If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• French translation
  anticorps

• Gene target
  LC3   APG8   166AT1234  
• Gene symbol
  MAP1LC3A
• Short name
  LC3 (APG8) Antibody (Clone 166AT1234)
• Technique
  Antibody, antibodies against human proteins, antibodies for
• Isotype
  Mouse IgG1-Kappa
• Alternative name
  LC3 (APG8) (antibody to-) (clonality 166AT1234)
• Alternative technique
  antibodies
• Clone name
  166AT1234

Gene info

• Identity
  HGNC:6838
• Gene
  MAP1LC3A
• Long gene name
  microtubule associated protein 1 light chain 3 alpha
• Synonyms
  • MAP1BLC3
  • MAP1ALC3
  • LC3
  • LC3A
  • ATG8E
• Locus
  20q11.22
• Discovery year
  2000-06-29
• Entrez gene record
  84557
• Pubmed identfication
  • 8833088
  • 17580304
• RefSeq identity
  • NM_181509
• Classification
  • Autophagy related
• VEGA ID
  OTTHUMG00000032306

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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