ERK1/2 (Phospho-T202) polyclonal antibody

• Catalog number
  BS94043-50ul
• Price
  Please ask
• Size
  50ul

• Alternative Name
  MAP kinase 3, MAPK 3, ERK-1, p44-MAPK, MAPK3, ERK1, PRKM3, MAP kinase 1, MAPK 1, ERK-2, p42-MAPK, MAP kinase 2, MAPK 2, MAPK1, ERK2, PRKM1, PRKM2,
• Immunogen
  Synthetic phospho-peptide corresponding to residues surrounding Thr185 of human Erk2.
• Swiss Prot
  P27361(Human) P28482(Human)
• Reactivity
  Human
• Applications
  WB ICC IHC FC
• Application_all
  WB:1:1,000 ICC:1:100-1:500 IHC:1:50-1:200 FC:1:50-1:100
• Product
  Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Purification Purity
  ProA affinity purified
• Storage Stability
  Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Specificity
  ERK1/2 (Phospho-T202) polyclonal antibody detects endogenous levels of ERK1/2 protein only when phosphorylated at T202.
• Usage
  ICC staining Phospho-Erk1(T202)+Erk2(T185) in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
• BiowMW
  42/44 kDa
• Note
  For research use only, not for use in diagnostic procedure.
• Properties
  If you buy Antibodies supplied by Bioworld they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• Group
  Polyclonals and antibodies
• About
  Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
• French translation
  anticorps

• Gene target
  ERK1/2   Phospho-T202  
• Gene symbol
  MAPK3
• Short name
  ERK1/2 (Phospho-T202) polyclonal antibody
• Technique
  Polyclonal, Antibody, antibodies against human proteins, antibodies for, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
• Host
  Rabbit
• Alternative name
  ERK1/2 (phosphorilated-T202) polyclonal (antibody to-)
• Alternative technique
  polyclonals, antibodies

Gene info

• Identity
  HGNC:6877
• Gene
  MAPK3
• Long gene name
  mitogen-activated protein kinase 3
• Synonyms gene
  • PRKM3
• Synonyms
  • ERK1
  • p44mapk
  • p44erk1
• GenBank acession
  • M84490
• Locus
  16p11.2
• Discovery year
  1993-11-05
• Entrez gene record
  5595
• Pubmed identfication
  • 9628824
• Classification
  • Mitogen-activated protein kinases
• VEGA ID
  OTTHUMG00000132149

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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