Form/Appearance
FITC (FITC conjugated Ig buffered in PBS, 0.02% NaN3 and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/ml. (Purified from ascitic fluid via Protein G Chromatography))
Concentration
Antibody Concentration: 0.1 miligram per 1 mililiter
Storage and shipping
Store the antibody at +4 degrees Celsius. For long term storage, aliquot and freeze unused portion the antibody should be stored at -20 degrees Celsius. in volumes appropriate for single usage. Avoid freeze/thaw cycles.
Tested applications:
Immunohistochemistry (IHC)
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Properties
This MBS Monoclonals Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MBS Monoclonals FITC is highly sensitive to pH extremes.
Conjugation
Anti-FITC Antibody
Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
Latin name
Mus musculus,Rattus norvegicus
About
Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
Gene targetRT1.Bu
OX-3
IgG1
Short name
Anti- RT1.Bu FITC (Clone OX-3) (mouse IgG1)
Technique
Mouse, anti, FITC
Isotype
IgG1
Label
FITC
Alternative name
Anti-Anti-Rat RT1.Bu FITC (Clone OX-3) (mouse IgG1)
Alternative technique
antibodies
Identity 4847
Gene HCRT
Long gene name hypocretin neuropeptide precursor
Synonyms gene name- hypocretin (orexin) neuropeptide precursor
Locus 17q21.2
Discovery year 1998-04-21
Entrez gene record 3060
Havana BLAST/BLAT OTTHUMG00000180107
