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Synonyms
CCN1, G1G1 protein
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Alternative_names
CCN1, G1G1 protein
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Description
Induces angiogenesis by stimulating the proliferation, migration, and adhesion of endothelial cells
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Recombinant
Yes
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Source
E. coli.
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Purity by SDS PAGE
≥95%
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Assay
SDS-PAGE
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Endotoxin Level
< 0.1 ng/ug of protein (
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Activity Specifications test method
Determined by the dose-dependent stimulation of the proliferation of murine 3T3 cells. The expected ED50 for this effect is 2.0-3.0 ug/ml.
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Biological activity
Determined by the dose-dependent stimulation of the proliferation of murine 3T3 cells. The expected ED50 for this effect is 2.0-3.0 ug/ml.
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Molecular Weight
39.4 kDa
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Storage Temp
-20°C
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Shipping
Gel Pack
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Shelf Life
12 months
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Appearance
Lyophilized powder
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Physical form description
Sterile filtered through a 0.2 micron filter. Lyophilized from 10 mM Sodium Citrate, pH 3.0 and 50 mM NaCl.
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Reconstitution Instructions
Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not raise to neutral pH. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
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Background Information
CYR61 is a member of the CCN family of secreted cysteine rich regulatory proteins. CYR61 induces angiogenesis by stimulating the proliferation, migration, and adhesion of endothelial cells. Cell migration and adhesion are mediated through binding to specific cell surface integrins and to heparin sulfate proteoglycans. Increased expression of CYR61 is associated with several types of cancer, and correlates with the progression and estrogen independence of human breast cancers. Recombinant human CYR61 is a 39.4 kDa protein containing 357 amino acid residues. It is composed of four distinct structural domains (modules); the IGF binding protein (IGFBP) domain, the von Willebrand Factor C (VWFC) domain, the Thrombospondin type-I (TSP type-1) domain, and a C-terminal cysteine knot-like domain (CTCK).
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Amino acid sequence
MTCPAACHCP LEAPKCAPGV GLVRDGCGCC KVCAKQLNED CSKTQPCDHT KGLECNFGAS STALKGICRA QSEGRPCEYN SRIYQNGESF QPNCKHQCTC IDGAVGCIPL CPQELSLPNL GCPNPRLVKV TGQCCEEWVC DEDSIKDPME DQDGLLGKEL GFDASEVELT RNNELIAVGK GSSLKRLPVF GMEPRILYNP LQGQKCIVQT TSWSQCSKTC GTGISTRVTN DNPECRLVKE TRICEVRPCG QPVYSSLKKG KKCSKTKKSP EPVRFTYAGC LSVKKYRPKY CGSCVDGRCC TPQLTRTVKM RFRCEDGETF SKNVMMIQSC KCNYNCPHAN EAAFPFYRLF NDIHKFRD
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Handling
Centrifuge the vial prior to opening.
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Usage
For Research Use Only! Not to be used in humans
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Additional source
Recombinants or rec. proteins
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Group
recombinants
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Gene target
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Gene symbol
CCN1
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Short name
CYR61, Recombinant
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Technique
Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
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Species
Human, Humans
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Alternative name
cysteine-rich, angiogenic inducer, 61, H. sapiens Rec.
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Alternative technique
rec
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Alternative to gene target
cysteine-rich, angiogenic inducer, 61, CCN1 and GIG1 and IGFBP10, CYR61 and IDBG-100054 and ENSG00000142871 and 3491, extracellular matrix binding, Extracellular, Cyr61 and IDBG-198706 and ENSMUSG00000028195 and 16007, CYR61 and IDBG-637282 and ENSBTAG00000009844 and 508941
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