This antibody has been succesfully tested for use in following methods:
Target of this antibody:
Formulation of this antibody:
In PBS with 0.05% (W/V) sodium azide.
Alternative names of antibody target:
Shelf time: 12 months in temp.: -20 deg.C
This antibody reacts with:
Clonality of antibody:
Polyclonal antibody raised in rabbit against human JARID2 (jumonji, AT rich interactive domain 2) using two KLH conjugated synthetic peptides containing a sequence from the N-terminal and the central region of the protein, respectively.
How to handle this antibody:
The antibody solution should be gently mixed before use.
JARID2 is a regulator of histone methyltransferase complexes that plays an essential role in embryonic development, including heart and liver development, neural tube fusion process and hematopoiesis. JARID2 acts by recruiting histone methyltransferase complexes (PRC2) to their target genes and modulating the histone methyltransferase activity. It does not have histone demethylase activity but regulates activity of various histone methyltransferase complexes. In embryonic stem cells, it associates with the PRC2 complex and inhibits trimethylation of H3K27me3 by the PRC2 complex, thereby playing a key role in differentiation of embryonic stem cells and normal development. In cardiac cells, it is required to repress expression of cyclin-D1 (CCND1) by activating H3K9 methylation by the GLP1/EHMT1 and G9a/EHMT2 histone methyltransferases.
Form of antibody:
Concentration of antibody:
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
Polyclonals and antibodies
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
anti-JARID2 polyclonal antibody
Polyclonal, Antibody, anti
antibody to-jumonji, AT rich interactive domain 2 polyclonal (Antibody to)
Alternative to gene target
jumonji, AT rich interactive domain 2, JMJ, JARID2 and IDBG-62820 and ENSG00000008083 and 3720, protein binding, nuclei, Jarid2 and IDBG-147776 and ENSMUSG00000038518 and 16468, JARID2 and IDBG-636025 and ENSBTAG00000012938 and 528253