DNA Binding Protein-7 (DBP-7), human recombinant
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Catalog number7603-100
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PricePlease ask
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Size100 ug
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SynonymsDBP, DNA Binding Protein-7 (DBP-7), human recombinant
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Alternative_namesChromodomain-helicase-DNA-binding protein 7 (CHD-7) (EC 3.6.4.12) (ATP-dependent helicase CHD7)
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DescriptionPromotes annealing of complementary DNA strands
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RecombinantYes
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SourceE. coli
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Purity by SDS PAGE≥99%
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AssaySDS-PAGE
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Molecular Weight9.44 kDa
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Storage Temp-20°C
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Shippinggel pack
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Shelf Life12 months
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AppearanceLyophilized protein
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Physical form descriptionSterile filtered and lyophilized with no additives
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Reconstitution InstructionsReconstitute in ddH₂O to a concentration of 1.0 mg/ml. Aliquot and store at –20°C for future use. Repeated freeze/thaw cycles should be avoided.
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Background InformationDNA Binding Protein-7 (DBP-7) is a N-terminal His-tagged recombinant protein and a member of the Sso7d family of small, abundant, non-specific DNA-binding proteins from the hyperthermophilic Archea Sulfolobus. The 7-kDa protein from Sulfolobus spp. consists of a five stranded, incomplete β-barrel capped at the opening by a C-terminal α-helix; they bind to the minor groove of a DNA duplex via the triple-stranded β-sheet. The topology of the Sulfolobus 7-kDa proteins was found to be similar to that of chromatin organization modifier (chromo) domains and eukaryotic SH3 domains, which are involved in protein–protein interactions. In vitro studies have shown that DBP-7 promotes the annealing of complementary DNA strands, induces negative supercoiling and chaperones the disassembly and renaturation of protein aggregates in an ATP hydrolysis-dependent manner.
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HandlingCentrifuge the vial prior to opening.
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UsageFor Research Use Only! Not to be used in humans
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PropertiesHuman proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Additional sourceRecombinants or rec. proteins
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Grouprecombinants
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Gene target
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Gene symbolDBP, DDX1, GC, PIRC34, PIRC33, PRKDC, PIRC32, SNORD114-7, IGHV3OR15-7, SNORD115-7
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Short nameDNA Binding Protein-7 (DBP-7), recombinant
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TechniqueRecombinant, dna, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
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SpeciesHuman, Humans
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Alternative nameDesoxyribonucleic acid Binding Protein-7 (DBP-7), H. sapiens Rec.
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Alternative techniquerec
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Gene info
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Identity
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Gene
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Long gene nameD-box binding PAR bZIP transcription factor
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Synonyms gene name
- D site of albumin promoter (albumin D-box) binding protein
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Synonyms
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GenBank acession
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Locus
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Discovery year1991-11-27
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- PAR bZIP family
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameDEAD-box helicase 1
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Synonyms gene name
- DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 1
- DEAD (Asp-Glu-Ala-Asp) box polypeptide 1
- DEAD (Asp-Glu-Ala-Asp) box helicase 1
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1993-07-06
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- DEAD-box helicases
- tRNA splicing ligase complex
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameGC vitamin D binding protein
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Synonyms gene name
- group-specific component (vitamin D binding protein)
- GC, vitamin D binding protein
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Synonyms
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GenBank acession
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Locus
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Discovery year1986-01-01
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Entrez gene record
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Pubmed identfication
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namepiwi-interacting RNA cluster 34
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Locus
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Discovery year2009-11-05
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Entrez gene record
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Pubmed identfication
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Classification
- Piwi-interacting RNA clusters
Gene info
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Identity
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Gene
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Long gene namepiwi-interacting RNA cluster 33
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Locus
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Discovery year2009-11-05
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Entrez gene record
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Pubmed identfication
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Classification
- Piwi-interacting RNA clusters
Gene info
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Identity
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Gene
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Long gene nameprotein kinase, DNA-activated, catalytic subunit
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Synonyms gene
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Synonyms gene name
- protein kinase, DNA-activated, catalytic polypeptide
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Synonyms
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Synonyms name
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Locus
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Discovery year1993-11-09
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Armadillo like helical domain containing
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VEGA ID
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Locus Specific Databases
Gene info
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Identity
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Gene
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Long gene namepiwi-interacting RNA cluster 32
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Locus
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Discovery year2009-11-05
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Entrez gene record
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Pubmed identfication
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Classification
- Piwi-interacting RNA clusters
Gene info
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Identity
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Gene
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Long gene namesmall nucleolar RNA, C/D box 114-7
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Synonyms
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Locus
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Discovery year2006-09-26
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Small nucleolar RNAs, C/D box
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin heavy variable 3/OR15-7 (pseudogene)
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Synonyms gene name
- immunoglobulin heavy variable 3/OR15-7
- immunoglobulin heavy variable 3/OR15-7 pseudogene
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Synonyms
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GenBank acession
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Locus
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Discovery year2000-04-17
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Entrez gene record
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Classification
- Immunoglobulin heavy (IGH) orphons
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namesmall nucleolar RNA, C/D box 115-7
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Synonyms
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GenBank acession
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Locus
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Discovery year2007-02-15
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Small nucleolar RNAs, C/D box
MeSH Data
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Name
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ConceptScope note: The initial culturing of cells derived directly from fresh TISSUES.
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Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
