Antibody's full description
Rabbit Anti-Human BIM/BOD (IN) Polyclonal
Antibody's other name
BCL2 like protein 11 Antibody, Bcl2 interacting mediator of cell death Antibody, Bcl2 interacting protein Bim Antibody, BCL2 like 11 Antibody, BAM Antibody, BimEl Antibody, BimL Antibody, BOD Antibody, BIM beta 6 Antibody, BIM Beta7 Antibody, BIM alpha 3 Antibody, BIM alpha4 Antibody, BIM alpha5 Antibody, BIM alpha6 Antibody
Antibody's recommended dilutions for use
WB (1:1000), IHC (1:100); optimal dilutions for assays should be determined by the user.
Certificate of analysis
1 µg/ml of SPC-113 was sufficient for detection of Bim in 20 µg of K562 and A549 cell lysates by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.
Members in the Bcl-2 family are critical regulators of apoptosis by either inhibiting or promoting cell death. Bim/BOD is a group of three splice variants, BimEL, BimL and BimS, with apparent molecular masses of ~23, 16, and 13 kDa, respectively. Bcl-2 homology 3 (BH3) domain is a potent death domain. BH3 domain containing pro-apoptotic proteins, including Bad, Bax, Bid, Bik, and Hrk, form a growing subclass of the Bcl-2 family. A novel BH3 domain containing protein was recently identified and designated Bim or BOD in human, mouse and rat (1,2). Bim/BOD interacts with diverse members in the pro-survival Bcl-2 sub-family including Bcl-2, Bcl-xL and Bcl-w. Bim/BOD induces apoptosis. The messenger RNA of Bim is ubiquitously expressed in multiple tissues and cell lines (1 ,2).
1. O'Connor L., Strasser A., O'Reilly L.A., et al. (1998) EMBO J. 17: 384-395. 2. Hsu S.Y., Lin P., and Hsueh A.J. (1998) Mol. Endocrinol. 12: 1432-40.
Representative figure legend
Immunohistochemistry analysis using Rabbit Anti-BIM Polyclonal Antibody (SPC-113). Tissue: skin cancer cells. Species: Human. Primary Antibody: Rabbit Anti-BIM Polyclonal Antibody (SPC-113) at 1:100. | Western blot analysis of Human K562 (Left) and A549 (Right) whole cell lysates showing detection of BIM protein using Rabbit Anti-BIM Polyclonal Antibody (SPC-113). Primary Antibody: Rabbit Anti-BIM Polyclonal Antibody (SPC-113) at 1:1000. Rabbit Anti-BIM Antibody used in Immunohistochemistry (IHC) on Human skin cancer cells (SPC-113) | Rabbit Anti-BIM Antibody used in Western blot (WB) on Human K562 (Left) and A549 (Right) whole cell lysates (SPC-113)
Net weight (g)
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
StressMark antibodies supplies antibodies that are for research of human proteins.
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Polyclonals and antibodies
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.