Granzyme A, human recombinant
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Catalog number4279-1000
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PricePlease ask
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Size1 mg
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SynonymsCTL tryptase, Cytotoxic T-lymphocyte proteinase 1, Fragmentin-1, Granzyme-1, Hanukkah factor (H factor, HF)
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Alternative_namesCTL tryptase, Cytotoxic T-lymphocyte proteinase 1, Fragmentin-1, Granzyme-1, Hanukkah factor, H factor, HF
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DescriptionSerine proteases which induce apoptosis
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RecombinantYes
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SourceMammalian cell expression system
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Purity by SDS PAGE≥95%
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AssaySDS-PAGE
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Purity≥95%
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Molecular Weight26.9 kDa
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Storage Temp-20°C
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ShippingGel pack
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Shelf Life12 months
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AppearanceLyophilized protein
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Physical form descriptionLyophilized from a 0.2 um filtered solution of 20 mM MES and150 mM NaCl, pH 5.5.
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Reconstitution InstructionsDissolve in 1x PBS (It is not recommended to reconstitute to a final concentration less than 100 ug/ml.). This can further be diluted to other aqueous buffers.
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Background InformationGranzyme A is a member of the granzyme family. Granzyme A is the most abundant serine protease in cytotoxic T lymphocytes (CTL) and natural killer (NK) cells. Granzyme A has a specific function in CTL and NK cells. It induces caspase-independent cell death when introduced into target cells by perforin. Human granzyme A is synthesized as a precursor (262 residues) with a signal peptide (residues 1-26), a propeptide (residues 27-28) and a mature chain (residues 29-262).
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Amino acid sequenceRecombinant Human Granzyme A is produced with mammalian cells expression system. Target protein is expressed with sequence (Glu27-Val262) of Human granzyme a (Uniprot #P12544) fused with a polyhistidine tag at the C-terminus.
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HandlingCentrifuge the vial prior to opening.
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UsageFor Research Use Only! Not to be used in humans
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PropertiesHuman proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Additional sourceRecombinants or rec. proteins
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Grouprecombinants
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Gene target
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Gene symbolGZMAP1, GZMA, SET
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Short nameGranzyme A, recombinant
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TechniqueRecombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
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SpeciesHuman, Humans
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Alternative nameGranzyme A, H. sapiens Rec.
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Alternative techniquerec
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Gene info
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Identity
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Gene
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Long gene namegranzyme A pseudogene 1
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Synonyms gene name
- granzyme A (granzyme 1, cytotoxic T-lymphocyte-associated serine esterase 3) pseudogene 1
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Locus
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Discovery year2012-06-29
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namegranzyme A
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Synonyms gene
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Synonyms gene name
- granzyme A (granzyme 1, cytotoxic T-lymphocyte-associated serine esterase 3)
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Synonyms name
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Locus
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Discovery year1988-05-31
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Granule associated serine proteases of immune defence
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameSET nuclear proto-oncogene
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Synonyms gene name
- SET translocation (myeloid leukemia-associated)
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1998-04-20
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- INHAT complex
- SET complex
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VEGA ID
MeSH Data
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Name
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ConceptScope note: The initial culturing of cells derived directly from fresh TISSUES.
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Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
