Mca-RPPGFSAFK(Dnp)

Alternative_name

Protease substrate; Mca - RPPGFSAF - K(Dnp)
Description

A useful fluorescence resonance energy transfer (FRET) substrate for assaying ECE-1 and ECE-2, ACE1 and ACE2. neprilysin, thermolysin (TLN), MMP-2 and MMP9, thimet oligopeptidase, cathepsin A, cathepsin X/Z, BACE, and insulinase, but not MMP1. Fluorescence of Mca (donor) is quenched by Dnp (quencher) in the intact peptide. Proteolytic cleavage of the FRET peptide separates the donor and the quencher, recovering the quenched fluorescence of Mca that is directly related to the enzyme activities and can be monitored at Ex/Em: 328 nm/393 nm
Structure Available

No
Peptide sequence

Mca-Arg-Pro-Pro-Gly-Phe-Ser-Ala-Phe-Lys(Dnp)-OH
Salt Form

TFA
Molecular Formula

C₆₆H₈₁N₁₅O₁₉
Molecular Weight

1388.46
Purity

≥95% by HPLC
Solubilities

DMSO (>10 mg/ml)
Handling

Protect from air and light
Tag Line

A protease substrate
Storage Condition

-20°C
Shipping Condition

Gel pack
Shelf Life

36 months
SMILES

N/A
InChi

N/A
InChi Key

N/A
Data Sheet Link
MSDS Available

Yes