Bcl-2 Antibody (Clone Bcl-2/100)
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Catalog number
3195-100
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Price
Please ask
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Size
100 µg
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Antibody Target
Bcl-2
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Target Alternative Name
BCL2, BCL 2
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Antibody Type
Monoclonal
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Clone
Bcl-2/100
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Species Reactivities
Human
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Immunogen Sequence
Synthetic peptide corresponding to resides surrounding a.a. 44 of Human Bcl-2
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Accession
M13994
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Gene Id
596
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Appearance
Colorless liquid
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Form
Liquid
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Concentration
0.5 mg/ml
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Formulation
100 µg (0.5 mg/ml) protein A purified from (low FBS containing) tissue culture supernatant. Purity was >95%. The antibody was formulated in PBS containing 1 mg/ml BSA and 1.5 mM sodium azide and 50% glycerol
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Purification
Affinity purified
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Application
Western blot, Immunoprecipitation, Immunohistochemistry
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Application And Usages
Western blot (1-4 µg/ml), Immunoprecipitation, Immunohistochemistry, and Flow cytometry. However the optimal conditions should be determined individually. Recognize the 26 kDa human Bcl-2
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Description
Bcl-2 (B-cell leukemia/lymphoma 2) gene was discovered by walking the chromosomal breakpoints t(14;18). This gene consists of two exons and several transcripts that encode two different protein products, Bcl-2α and Bcl-2β. Both proteins are approximately 26-30 kDa and differ at their C-terminal region. Bcl-2 suppresses apoptosis (programmed cell death) and this property appears to be modulated by homodimerization or association with Bax and Bad, two cell death promoters
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Storage Condition
-20 °C
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Shipping Condition
gel pack
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Shelf Life
12 months
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Usage
For Research Use Only! Not to be used in humans.
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Handling
The antibody solution should be gently mixed before use.
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Western Blot Verified
Verified
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Immunocytochemistry Verified
Not Verified
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Immunofluorescence Verified
Not Verified
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Immunoprecipitation Verified
Not Verified
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ELISA Verified
Not Verified
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ChIP Verified
Not Verified
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Flow Cytometry Verified
Not Verified
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Properties
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translation
anticorps
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Gene target
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Short name
Bcl-2 Antibody (Clone Bcl-2/100)
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Technique
Antibody, antibodies against human proteins, antibodies for
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Isotype
Mouse IgG1
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Alternative name
Bcl-2 (antibody to-) (clonality Bcl-2/100)
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Alternative technique
antibodies
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Clone name
Bcl-2
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MeSH Data
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Name
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Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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