Rat anti-Mouse CD8 Monoclonal Antibody, FITC

• Catalog number
  E16MF008-100
• Price
  Please ask
• Size
  100 Tests

• Conjugation
  Unconjugated
• Note
  For research use only.
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use. Cytotoxic T cells cluster differentiators for flow cytometry. This transmembrane glycoprotein is detected by monoclonals and has a lot of clones available with different affinity.
• Properties
  If you buy Antibodies supplied by EnoGene they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This EnoGene Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. EnoGene FITC is highly sensitive to pH extremes.
• Additional conjugation
  Anti-FITC Antibody
• About
  Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes. Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
• Test
  Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
• Latin name
  Mus musculus, Rattus norvegicus
• French translation
  anticorps

• Gene target
  CD8   Monoclonal  
• Gene symbol
  CD8A
• Short name
  anti-Mouse CD8 Monoclonal Antibody, FITC
• Technique
  Antibody, anti-, Mouse, anti, FITC, antibody to, antibodies, antibodies against human proteins, antibodies for, Fluorescein, Monoclonals or monoclonal antibodies, mouses
• Host
  Rat
• Label
  FITC
• Species
  Mouse, Mouses, Rats
• Alternative name
  Rat Antibody toMouse CD8 monoclonal (antibody to-), fluorecein
• Alternative technique
  antibodies, murine, fluorescine

Gene info

• Identity
  HGNC:1706
• Gene
  CD8A
• Long gene name
  CD8a molecule
• Synonyms gene
  • CD8
• Synonyms gene name
  • CD8 antigen, alpha polypeptide (p32)
  • T-cell surface glycoprotein CD8 alpha chain
• Locus
  2p11.2
• Discovery year
  1986-01-01
• Entrez gene record
  925
• Pubmed identfication
  • 1541829
• RefSeq identity
  • NM_001768
• Classification
  • V-set domain containing
  • CD molecules
• VEGA ID
  OTTHUMG00000130265
• Locus Specific Databases
  • CD8Abase: Mutation registry for CD8 deficiency
  • LRG_44

MeSH Data

• Name
  Fluorescent Antibody Technique
• Concept
  Scope note: Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
• Tree numbers
  • E01.370.225.500.607.512.240
  • E01.370.225.750.551.512.240
  • E05.200.500.607.512.240
  • E05.200.750.551.512.240
  • E05.478.583.375
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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