Phospho-Akt2 (Ser474) Antibody

• Catalog number
  A1246-100
• Price
  Please ask
• Size
  100 µl

• Antibody Target
  AKT2
• Target Alternative Name
  AKT2; RAC-beta serine/threonine-protein kinase; Protein kinase Akt-2; Protein kinase B beta; PKB beta; RAC-PK-beta
• Antibody Type
  Polyclonal
• Species Reactivities
  Human, mouse , rat
• Immunogen Sequence
  Synthesized peptide derived from human Akt2 around the phosphorylation site of S474
• Accession
  P31751
• Gene Id
  208
• Appearance
  Colorless liquid
• Form
  Liquid
• Formulation
  In PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide
• Purification
  Affinity-chromatography using epitope-specific immunogen
• Application
  WB, ELISA, IHC
• Positive Control
  WB: Mouse brain lysate, 293 cell lysate
• Application And Usages
  WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:20000
• Description
  AKT2 is a putative oncogenic protein belonging to a subfamily of serine/threonine kinases containing SH2-like (Src homology 2-like) domains. Furthermore, AKT2 was shown to be amplified and overexpressed in 2 of 8 ovarian carcinoma cell lines and 2 of 15 primary ovarian tumors. Overexpression of AKT2 contributes to the malignant phenotype of a subset of human ductal pancreatic cancers. AKT2 is a general protein kinase capable of phophorylating several known proteins.
• Storage Condition
  -20°C
• Shipping Condition
  gel pack
• Shelf Life
  12 months
• Usage
  For Research Use Only! Not to be used in humans
• Handling
  The antibody solution should be gently mixed before use
• Western Blot Verified
  Verified
• Immunocytochemistry Verified
  Not Verified
• Immunofluorescence Verified
  Not Verified
• Immunoprecipitation Verified
  Not Verified
• FACS Verified
  Not Verified
• ELISA Verified
  Not Verified
• ChIP Verified
  Not Verified
• Dot Blot Verified
  Not Verified
• Flow Cytometry Verified
  Not Verified
• Data Sheet Link
• Properties
  If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• French translation
  anticorps

• Gene target
  Phospho-Akt2   Ser474  
• Short name
  Phospho-Akt2 (Ser474) Antibody
• Technique
  Antibody, antibodies against human proteins, antibodies for
• Isotype
  Rabbit IgG
• Alternative name
  phosphorilated-v-akt murine thymoma viral oncogene homolog 2 (Ser474) (antibody to-)
• Alternative technique
  antibodies
• Alternative to gene target
  v-akt murine thymoma viral oncogene homolog 2, HIHGHH and PKBB and PKBBETA and PRKBB and RAC-BETA, AKT2 and IDBG-51270 and ENSG00000105221 and 208, transferase activity, nuclei, Akt2 and IDBG-161456 and ENSMUSG00000004056 and 11652, AKT2 and IDBG-637808 and ENSBTAG00000001400 and 534923

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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