Mouse Monoclonal IgG3 Anti-CA 15-3 (CD227, MUC-1) [C595(NCRC48)]
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Catalog numberCA153-1
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PricePlease ask
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Size1 ml
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AvailabilityPlease contact us to check the availability
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ImmunogenPurified urinary MUC-1
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SpecificityHuman mamma carcinoma CA 15-3 (MUC-1, CD227). No cross-reactivity to other tumor-associated antigens like AFP, CEA, PAP, PSA, CA19-9 and CA125.
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Species reactivityHuman
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Product presentationAntibody solution in stabilizing phosphate buffer pH 7.3. Contains 0.09 % sodium azide**. The volume is sufficient for at least 100 immunohistochemical tests (100 µl working solution / test). Use appropriate antibody diluent e.g. Gentaur Art .No. PU002.
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Product categoryPrimary Antibody
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Antibody s raised inMouse
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Antibody s isotypeIgG3
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Antibody s cloneC595(NCRC48)
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Product is supplied asliquid concentrate
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Recommended concentration for use1:20 - 1:100
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Protein concentration100 µg/ml
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Positive controlMamma carcinoma
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PretreatmentIn paraffin section no pre-treatment is required, however heat induced antigen retrieval may improve the staining. Each laboratory should select it's own optimum staining and pre-treatment conditions.
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Tested applicationsIHC(C, P), ELISA, WB
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Recommended secondary reagentWe recommend the use of Gentaur's Universal Staining System DAB (Art. No. DA005) or AEC (Art.-No. AE005), if higher sensitivity is required VECTASTAIN Elite ABC (Art. No. PK-6102) or ABC AP (Art. No. AK-5002) systems are applicable.
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Storage methodStore at 2-8°C
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UniProt numberP15941 MUC1_Human
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Litterature1. Price, M.R. et al. (1990). Immunological and structural features of the protein core of human polymorphic epithelial mucin. Molec. Immunol. 27: 795-802. 2. Price, M. R. et al. (1990). C595 - a monoclonal antibody against the protein core of human urinary epithelial mucin commonly expressed in breast carcinomas. Br. J. Cancer. 61: 681. 3. Gatalica Z., and Miettienen M. (1994) Distribution of carcinoma antigens CA 19-9 and CA 15-3: an immunohistochemical study of 400 tumors. Appl. Immunohistochem. 2(3); 205ff. 4. Denton, G. et al. (1995). Primary sequence determination and molecular modelling of the variable region of an anti MUC1 mucin monoclonal antibody. Eur. J. Cancer 31A: 214-221. 5. Denton, G. et al. (1997). Production and characterization of a recombinant anti Muc 1 scFv reactive with human carcinomas. Br. J. Cancer. 76 (5): 614-21.
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Tips*Gentaur's antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals. ** The preservative sodium azide is known to be poisonous and potentially hazardous to health. It should be handled only by trained staff. Despite of the product's low azide concentration it must be handled with care. Dispose according to regional rules!
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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AboutMonoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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TestMouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin nameMus musculus
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Gene target
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Gene symbolMUC1, IGHVII-15-1, ALOX15, PIRC66, PIRC71, PIRC75, PIRC67, PIRC72, PIRC68, PIRC62
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Short nameResearch
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TechniqueMouse monoclonal, anti-, Mouse, anti, antibody to, antibodies, Monoclonals or monoclonal antibodies, mouses, mouse monoclonals
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Hostmouse monoclonal, Murine monoclonal antibodies or Mabs are often conjugated and the isotype is IgG.
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IsotypeIgG3
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SpeciesMouse, Mouses
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Alternative nameCA 15-3 (CD227, MUC-1)
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Alternative techniquemurine, antibodies
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Gene info
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Identity
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Gene
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Long gene namemucin 1, cell surface associated
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Synonyms gene
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Synonyms gene name
- mucin 1, transmembrane
- medullary cystic kidney disease 1 (autosomal dominant)
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1986-01-01
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- CD molecules
- Mucins
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin heavy variable (II)-15-1 (pseudogene)
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Synonyms gene name
- immunoglobulin heavy variable (II)-15-1
- immunoglobulin heavy variable (II)-15-1 pseudogene
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Synonyms
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GenBank acession
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Locus
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Discovery year2000-04-17
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin heavy locus at 14q32.33
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namearachidonate 15-lipoxygenase
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Synonyms
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GenBank acession
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Locus
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Discovery year1992-11-20
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Entrez gene record
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Pubmed identfication
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Classification
- Arachidonate lipoxygenases
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namepiwi-interacting RNA cluster 66
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Locus
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Discovery year2009-11-05
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Entrez gene record
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Pubmed identfication
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Classification
- Piwi-interacting RNA clusters
Gene info
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Identity
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Gene
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Long gene namepiwi-interacting RNA cluster 71
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Locus
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Discovery year2009-11-05
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Entrez gene record
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Pubmed identfication
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Classification
- Piwi-interacting RNA clusters
Gene info
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Identity
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Gene
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Long gene namepiwi-interacting RNA cluster 75
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Locus
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Discovery year2009-11-05
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Entrez gene record
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Pubmed identfication
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Classification
- Piwi-interacting RNA clusters
Gene info
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Identity
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Gene
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Long gene namepiwi-interacting RNA cluster 67
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Locus
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Discovery year2009-11-05
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Entrez gene record
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Pubmed identfication
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Classification
- Piwi-interacting RNA clusters
Gene info
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Identity
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Gene
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Long gene namepiwi-interacting RNA cluster 72
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Locus
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Discovery year2009-11-05
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Entrez gene record
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Pubmed identfication
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Classification
- Piwi-interacting RNA clusters
Gene info
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Identity
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Gene
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Long gene namepiwi-interacting RNA cluster 68
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Locus
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Discovery year2009-11-05
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Entrez gene record
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Pubmed identfication
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Classification
- Piwi-interacting RNA clusters
Gene info
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Identity
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Gene
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Long gene namepiwi-interacting RNA cluster 62
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Locus
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Discovery year2009-11-05
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Entrez gene record
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Pubmed identfication
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Classification
- Piwi-interacting RNA clusters
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
