BIG1/ARFGEF1 Antibody, ALEXA FLUOR 350

• Catalog number
  bs-12866R-A350
• Price
  Please ask
• Size
  100ul

• Long name
  BIG1/ARFGEF1 Polyclonal Antibody, ALEXA FLUOR 350 Conjugated
• Also known as
  Anti-BIG1/ARFGEF1 PAb ALEXA FLUOR 350
• Category
  Conjugated Primary Antibodies
• Conjugated with
  ALEXA FLUOR® 350
• Host Organism
  Rabbit (Oryctolagus cuniculus)
• Target Antigen
  BIG1/ARFGEF1
• Specificity
  This is a highly specific antibody against BIG1/ARFGEF1.
• Modification
  Unmodified
• Modification Site
  None
• Clonality
  Polyclonal
• Clone
  Polyclonal antibody
• Concentration
  1ug per 1ul
• Source
  This antibody was obtained by immunization of the host with KLH conjugated synthetic peptide derived from human BIG1/ARFGEF1
• Gene ID Number
  10565
• Tested applications
  IF(IHC-P)
• Recommended dilutions
  IF(IHC-P)(1:50-200)
• Crossreactivity
  Human, Mouse, Rat
• Cross reactive species details
  Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.
• Background of the antigen
  Guanine nucleotide-exchange proteins (GEPs) accelerate replacement of bound GDP with GTP and thereby activate ADP-ribosylation factors (ARFs), a family of guanine nucleotide-binding proteins that play an important role in intracellular vesicular trafficking. GEPs comprise two major families, large GEPs that are inhibited by brefeldin A (BFA), a protein that effects Golgi structure and a group of smaller GEPs that are insenstive to BFA. Two genes for GEPs found on human chromosomes 8 and 20 encode BFA sensitive GEPs designated BIG1 and BIG2. Both GEPS contain a sec7 domain that is responsible for their brefeldin inhibition and also their catalytic activity. In vivo, BIG1 and BIG2 exist in macromolecular complexes that move between the Golgi membranes and cytosol. BIG2 associates with PKA regulatory subunits, implying that BIG2 may act as an A kinase-anchoring protein (AKAP) that could coordinate the cAMP and ARF regulatory pathways.
• Purification
  Purified by Protein A.
• Storage conditions
  Store this antibody in aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Keep refrigerated at 2 to 8 degrees Celcius for up to one year.
• Excitation emission
  343nm/442nm
• Synonyms
  ADPribosylation factor guanine nucleotide exchange factor 1brefeldin A inhibited; ARFGEF1; ARFGEP1; Brefeldin A inhibited GEP 1; Brefeldin A inhibited guanine nucleotide exchange protein 1; p200 ARF GEP1; p200 ARF guanine nucleotide exchange factor; BIG1_HUMAN.
• Properties
  For facs or microscopy Alexa 1 conjugate. Alexa Fluor 350 conjugates can be used in multi-color flow cytometry with FACS's equipped with a second red laser or red diode. If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies. ALEXA FLUOR they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• Conjugation
  Alexa Fluor, ALEXA FLUOR 350
• French translation
  anticorps

• Gene target
  BIG1/ARFGEF1  
• Gene symbol
  ARFGEF1, ARFGEF1-DT
• Short name
  Anti-BIG1/ARFGEF1
• Technique
  Antibody, antibodies against human proteins, antibodies for
• Isotype
  Immunoglobulin G (IgG)
• Label
  ALEXA FLUOR 350
• Alternative name
  BIG1/ARFGEF1 Polyclonal Antibody, ALEXA FLUOR 350
• Alternative technique
  antibodies
• Alternative to gene target
  ADP-ribosylation factor guanine nucleotide-exchange factor 1 (brefeldin A-inhibited), ARFGEP1 and BIG1 and P200, ARFGEF1 and IDBG-24344 and ENSG00000066777 and 10565, protein kinase A regulatory subunit binding, nuclei, Arfgef1 and IDBG-133303 and ENSMUSG00000067851 and 211673, ARFGEF1 and IDBG-644666 and ENSBTAG00000014691 and 281022

Gene info

• Identity
  HGNC:15772
• Gene
  ARFGEF1
• Long gene name
  ADP ribosylation factor guanine nucleotide exchange factor 1
• Synonyms gene name
  • ADP-ribosylation factor guanine nucleotide-exchange factor 1 (brefeldin A-inhibited)
• Synonyms
  • DKFZP434L057
  • BIG1
  • ARFGEP1
  • p200
• Synonyms name
  • Brefeldin A-inhibited guanine nucleotide-exchange protein 1
• GenBank acession
  • AF084520
• Locus
  8q13.2
• Discovery year
  2004-08-09
• Entrez gene record
  10565
• Pubmed identfication
  • 10212200
  • 8917509
• RefSeq identity
  • NM_006421
• Classification
  • ARFGEF family
  • Armadillo like helical domain containing
• VEGA ID
  OTTHUMG00000164626

Gene info

• Identity
  HGNC:55237
• Gene
  ARFGEF1-DT
• Long gene name
  ARFGEF1 divergent transcript
• Locus
  8q13.2
• Discovery year
  2020-09-16
• Entrez gene record
  102724708
• RefSeq identity
  • NR_136223
• Classification
  • Divergent transcripts

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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