Bovine Serum Albumin – Fraction V, 30% Solution

• Catalog number
  7914-500
• Price
  Please ask
• Size
  500 ml

• Description
  30% solution of Pure Cohn Fraction V BSA, for multiple applications. BSA is prepared by utilizing the Cohn Cold Ethanol Precipitation Process. This process is optimal for preserving the nutrient factors critical to the applications of cell culture, leptospira and microbiological growth. BSA makes up approximately 60% of all proteins in animal serum. It is commonly used in cell culture protocols, particularly when protein supplementation is necessary and the other components of serum are unwanted. It aids in stabilization of enzymes and other proteins to prevent proteolysis and denaturation, especially when the proteins are present in low concentrations. In cell culture its main role is as a carrier of small molecules. Because of its negative charge, BSA binds water, salts, fatty acids, vitamins and hormones, then carries these bound components between tissues and cells. The binding capacity also makes BSA an effective scavenger for removing toxic substances, including pyrogens, from the medium. This 30% serological solution is a sterile, amber colored solution of concentrated bovine serum albumin.
• Product Highlights
  SOURCE: The raw material used to manufacture this lot was derived exclusively from USDA-regulated abattoirs located in the United States. The animals received ante-mortem and post-mortem inspection under a licensed veterinarian’s supervision and were certified at the time of slaughter as free of infectious or contagious diseases and injurious parasites. PURITY: ≥ 96% by SDS-PAGE analysis MOL. WEIGHT: ~ 66.43 kDa FORM: Amber colored solution ALBUMIN CONTENT: 29-32% pH: 7.0 ± 0.2 APPLICATIONS/ADVANTAGES: • A convenient nutrient concentrate used to formulate media for bacteria such as Leptospira, Rickettsiaceae, Mycobacteria, Treponema, and parasitic protozoans. • Can be used as a nutrient in growth media for the manufacture of veterinary vaccines and in the Treponema pallidum immobilization test. • Can serve as a stock solution for using BSA in immunodiagnostic assays. • Can be used as the standard solution for serological applications. • It is isotonic to human erythrocytes. • Available with and without 0.1% sodium azide.
• Storage Temp
  +4°C
• Shipping
  gel pack
• Shelf Life
  5 years
• Datasheet
• Notes
  For research use only. Not to be used for human or animal treatment or consumption.
• Additional description
  Azide Free Serum for cell culture FBS fetal bovine serum. Filtered serum for sterility. The polyclonal serum contains no red or white blood cells or clotting factors; it is the blood plasma not including the fibrinogens. Serum includes all proteins and antibodies not used in blood clotting (coagulation) and all the electrolytes, antibodies, antigens, hormones, and any exogenous substances like drugs and microorganisms. Plasmas on request. The volume (ml) in milliliters of buffered % w/v solutions at the medium pH are also used for DNA extraction Organic (Phenol-Chloroform) Extraction, Non-Organic (Proteinase K and Salting out), Chelex (Ion Exchange Resin) Extraction, EDTA or PBS aqueous.
• Group
  Sera
• About
  Serum should be stored at +5°C

• Gene target
  Albumin   Fraction  
• Short name
  Serum Albumin – Fraction V, 30%
• Technique
  serum
• Species
  Bos Taurus genes are available form the Bovine Genome database.
• Alternative name
  Bovine blood serum Albumin – Fraction V, 30% solution
• Alternative technique
  sera
• Tissue
  serum

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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