-
Available ordering format
Liquid
-
Immunogen
Purified rabbit IgG (H&L)
-
Raised in
Goat
-
Clonality
Polyclonal
-
Clone
Polyclonal
-
Purification
Affinity purified goat IgG
-
How to reconstitute
See included datasheet or contact our support service
-
Storage condition
2-8°C. Shelf life is 1 year from date of receipt. Prepare working dilution prior to use and then discard.
-
Verified applications
Contact Gentaur support center
-
Connected products
other secondary antibodies against biotinylated rabbit IgG (H&L)
-
Recommended dilutions for use
This conjugate is suitable for all immunoassay applications. The optimal working dilution should be determined by the investigator. Suggested starting dilution(s): 1:2000 - 1:20 000 for ELISA/Western blot 1:500 - 1:5 000 for Immunohistochemistry
-
Molecular weight expected аpparent
see datasheet
-
Verified reactivity
to be determined
-
Possible reactivity
to be determined
-
No reactivity
to be determined
-
Supplementary information
-
References
Refer to PubMed
-
Scientific context
Goat anti-rabbit IgG (H&L) is a secondary antibody conjugated to biotin, which binds to rabbit IgG (H&L) in immunological assays. Antibody is affinity purified using solid phase rabbit IgG (H&L).
-
Notes
Purity of this preparation is > 95% based on SDS-PAGE. Antibody concentration is 1.0 mg/ml (E 1% at 280 nm = 13.0). Antibody is supplied in 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2.1 % (w/v) B, Protease/IgG free. Contains 0.05% (w/v) sodium azide as preservative of bacterial growth.Based on immunoelectrophoresis, this antibody reacts with: heavy chains on rabbit IgG, light chains on all rabbit immunoglobulins. Based on immunoelectrophoresis, no reactivity is observed to: non-immunoglobulin rabbit serum proteins, human serum proteins.
-
Protein number
Refer to NCBI
-
TAIR number
Refer to NCBI
-
-
Properties
Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
-
Conjugation
Biotinylated
-
Latin name
Capra aegagrus hircus
-
Description
Azide Free Serum for cell culture FBS fetal bovine serum. Filtered serum for sterility. The polyclonal serum contains no red or white blood cells or clotting factors; it is the blood plasma not including the fibrinogens. Serum includes all proteins and antibodies not used in blood clotting (coagulation) and all the electrolytes, antibodies, antigens, hormones, and any exogenous substances like drugs and microorganisms. Plasmas on request.
-
Group
Sera
-
About
Serum should be stored at +5°C
