Available ordering format Liquid
Immunogen Purified Rabbit IgG, whole molecule
Purification Purified goat IgG
How to reconstitute See included datasheet or contact our support service
Storage condition Store undiluted liquid at 2-8°C. Shelf life is 1 year from date of receipt.
Verified applications ELISA (ELISA) , western blot (WB), immunohistochemistry (IHC)
Connected products AS09 602 | Goat anti-rabbit IgG (H&L) HRP conjugatedAS09 607 | Goat anti-rabbit IgG (H&L) AP conjugated
Recommended dilutions for use 1: 2000 - 20 000 (ELISA) and (WB), 1: 500 -1: 5000 (IHC)
Verified reactivity rabbit IgG heavy and light chains (H&L)
Possible reactivity rabbit IgG heavy and light chains (H&L)
No reactivity no confirmed exceptions from predicted reactivity known in the moment
Supplementary information No reactivity is observed to non-immunoglobulin rabbit serum proteins based in immunoelectrophoresis
References to be added when available
Scientific context Goat anti-rabbit IgG is a secondary antibody conjugated to biotin which binds to all rabbit immunoglobulins in immunological assays.
Notes Antibody concentration is 4.55 mg/ml and it has been afinity purified on solid phase rabbit IgG (H&L)Biotin conjugate is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. Contains 0.05 % (w/v) sodium azide as preservative.The linker length is 22.4 angstroms.
Properties Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
Latin name Capra aegagrus hircus