Goat, L), Biotin conjugated

• Catalog number
  AS09 608
• Price
  Please ask
• Size
  2 mg

• Available ordering format
  Liquid
• Immunogen
  Purified Rabbit IgG, whole molecule
• Raised in
  Goat
• Clonality
  Polyclonal
• Clone
  Polyclonal
• Purification
  Purified goat IgG
• How to reconstitute
  See included datasheet or contact our support service
• Storage condition
  Store undiluted liquid at 2-8°C. Shelf life is 1 year from date of receipt.
• Verified applications
  ELISA (ELISA) , western blot (WB), immunohistochemistry (IHC)
• Connected products
  AS09 602 | Goat anti-rabbit IgG (H&L) HRP conjugatedAS09 607 | Goat anti-rabbit IgG (H&L) AP conjugated
• Recommended dilutions for use
  1: 2000 - 20 000 (ELISA) and (WB), 1: 500 -1: 5000 (IHC)
• Molecular weight expected аpparent
  see datasheet
• Verified reactivity
  rabbit IgG heavy and light chains (H&L)
• Possible reactivity
  rabbit IgG heavy and light chains (H&L)
• No reactivity
  no confirmed exceptions from predicted reactivity known in the moment
• Supplementary information
  No reactivity is observed to non-immunoglobulin rabbit serum proteins based in immunoelectrophoresis
• References
  to be added when available
• Scientific context
  Goat anti-rabbit IgG is a secondary antibody conjugated to biotin which binds to all rabbit immunoglobulins in immunological assays.
• Notes
  Antibody concentration is 4.55 mg/ml and it has been afinity purified on solid phase rabbit IgG (H&L)Biotin conjugate is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. Contains 0.05 % (w/v) sodium azide as preservative.The linker length is 22.4 angstroms.
• Protein number
  Refer to NCBI
• TAIR number
  Refer to NCBI
• Hyperlink
• Properties
  Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
• Conjugation
  Biotinylated
• Latin name
  Capra aegagrus hircus

• Gene target
  conjugated  
• Short name
  Goat, L), conjugated
• Technique
  Goat, antibody Conjugates, goats
• Host
  Goat, The goat polyclonal antibodies are directed against human genes or rabbit or mouse IgGs (H +L) chain primary antibodies and conjugated for detection. It are very good secondary antibodies that are affinity purified and have a good affinity themselves too. Often KLH is coupled to the antigen so anti KLH can be present in the vial.
• Label
  Biotin
• Species
  Goats
• Alternative name
  caprine, L), biotinilated coupled
• Alternative technique
  caprine

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