ICAD Antibody

• Catalog number
  N1102-100UG
• Price
  Please ask
• Size
  0.1mg

• Category
  Antibody
• Concentration
  0.5 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Form
  Purified
• Conjugation
  Unconjugated
• Clone
  Polyclonal antibody
• Recognised antigen
  ICAD
• Host animal
  Rabbit (Oryctolagus cuniculus)
• Clonality
  Polyclonal (rabbit origin)
• Species reactivity
  Mouse (Mus musculus) ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
• Tested applications
  WB
• Recommended dilutions
  Western blot: 4-8ug/ml
• Notes
  Provided assay concentrations are suggestions only, antibody titration may be required for optimal results.
• Added buffer
  1X Phosphate Buffered Saline (PBS), pH 7.4
• Intented use
  This ICAD antibodyis to be used only for research purposes and not for diagnostics..
• Gene ID
  1676
• Purity
  Purified by protein A Affinity Chromatography
• Description
  During apoptosis, toxic or useless cells are broken down for removal. DFF, or DNA fragmentation factor, is a heterodimeric protein which mediates DNA fragmentation and chromatin condensation during apoptosis. DFFB is the active DFF subunit but remains inactive while bound to DFFA, also call ICAD (Inhibitor of caspase-activated DNase). Caspase-3 cleaves ICAD, which then disassociates from DFFB. The disassociation removes the inhibitory effects of ICAD, activating DFFB and beginning the DNA fragmentation process.
• Immunogen
  A recombinant protein fragment from human ICAD was used as the immunogen for this antibody.
• Storage
  Aliquot the antibody and store frozen at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized.
• Properties
  If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• French translation
  anticorps

• Gene target
  ICAD  
• Gene symbol
  DFFA
• Short name
  Anti-ICAD
• Technique
  Antibody, antibodies against human proteins, antibodies for
• Isotype
  Rabbit IgG
• Alternative name
  Antibodies to ICAD
• Alternative technique
  antibodies

Gene info

• Identity
  HGNC:2772
• Gene
  DFFA
• Long gene name
  DNA fragmentation factor subunit alpha
• Synonyms gene name
  • DNA fragmentation factor, 45 kD, alpha polypeptide
  • DNA fragmentation factor, 45kDa, alpha polypeptide
• Synonyms
  • DFF-45
  • DFF45
  • ICAD
  • DFF1
• Synonyms name
  • DNA fragmentation factor, 45 kD, alpha subunit
• GenBank acession
  • AF087573
• Locus
  1p36.22
• Discovery year
  1997-10-27
• Entrez gene record
  1676
• Pubmed identfication
  • 9605855
  • 9108473
• RefSeq identity
  • NM_004401
• VEGA ID
  OTTHUMG00000001909

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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