Protein A (FITC)
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Catalog number65C-CE0402
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PricePlease ask
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Size5 mg
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Product TypeProteins
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Product SubtypeConjugated Proteins
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Research AreaMiscellaneous
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Tag ConjugateNA
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Type1Binding Protein
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Form BufferLyophilized from 0.02M K3PO4, pH 7.2, with 0.15M NaCl, 10 mg/ml BSA, and 0.01% NaN3. Immunoglobulin and protease free.
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StorageStore at 4 deg C until reconstitution. Following reconstitution aliquot and freeze at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.
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ApplicationsELISA, FC, IF
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Shipping InfoBlue Ice
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PropertiesThis fitzgerald Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. fitzgerald FITC is highly sensitive to pH extremes.
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ConjugationAnti-FITC Antibody
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Gene target
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Gene symbolCENPA, VAPA
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Short nameProtein A (FITC)
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TechniqueFITC, Fluorescein
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LabelFITC
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Alternative nameProtein A (fluorecein)
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Alternative techniquefluorescine
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Gene info
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Identity
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Gene
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Long gene namecentromere protein A
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Synonyms gene name
- centromere protein A (17kD)
- centromere protein A, 17kDa
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1988-05-31
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Entrez gene record
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RefSeq identity
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Classification
- Constitutive centromere associated network
- H3 histones
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameVAMP associated protein A
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Synonyms gene name
- VAMP (vesicle-associated membrane protein)-associated protein A (33kD)
- VAMP (vesicle-associated membrane protein)-associated protein A, 33kDa
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Synonyms
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Locus
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Discovery year1999-03-19
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Entrez gene record
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Pubmed identfication
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
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Tree numbers
- E01.370.225.500.607.512.240
- E01.370.225.750.551.512.240
- E05.200.500.607.512.240
- E05.200.750.551.512.240
- E05.478.583.375
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data