Mouse anti Cytokeratin 16 / Keratin K16

• Catalog number
  MUB0351P
• Price
  Please ask
• Size
  0,1 mg

• Category
  Primary Antibodies
• Long description
  Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in epithelial tissues by at least 20 different polypeptides. They range in molecular weight between 40 kDa and 68 kDa and isoelectric pH between 4.9 – 7.8. The individual Cytokeratin polypeptides are numbered 1 to 20. The various epithelia in the Human body usually express Cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of matuRation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The Cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays.
• Antibody come from
  LL025 is a Mouse monoclonal IgG1 antibody derived by fusion of SP2/0-Ag14 murine myeloma cells with spleen cells from a Mouse immunized with a synthetic peptide corresponding to an amino acid sequence of the carboxyl terminus of Human Keratin 16.
• Other description
  Each vial contains 100 ul 1 mg/ml purified monoclonal antibody in PBS containing 0.09% sodium azide.
• Clone
  LL025
• Antigen antibody binding interaction
  Mouse anti Cytokeratin 16 / Keratin K16 Antibody
• Antibody is raised in
  Mouse
• Antibody s reacts with
  Human
• Antibody s reacts with these species
  This antibody doesn't cross react with other species
• Antibody s specificity
  Keratin 16 is specifically found in hyperprolifeRative squamous epithelium such as psoriatic lesions and squamous cell carcinomas, e.g. of the lung. It is expressed in suprabasal Keratinocytes of wounded epidermis, a situation of high cell turnover. LL025 reactivity is negative in, e.g. normal skin, breast and lung tissue and non-invasive breast carcinoma. However, 10% of the invasive breast carcinomas tested were diffusely or focally positive with this antibody.
• Research interest
  Cytoskeleton
• Application
  Immunohistochemistry (frozen),Immunohistochemistry (paraffin),Western blotting
• Antibody s suited for
  LL025 is suitable for immunoblotting (Western blot) and for immunohistochemistry on frozen tissue and paraffin embedded material. Optimal antibody dilution should be determined by titration; 1:25 – 1:50 for immunohistochemistry with avidin-biotinylated Horseradish peroxidase complex (ABC) as detection reagent. For use in paraffin embedded tissue sections high pressure retrieval at pH 8,0 is recommended.
• Storage
  Store at 4°C, or in small aliquots at -20°C.
• Relevant references
  Wetzels, R.H.W., Kuijpers H.J.H., Lane, E.B., Leigh, I.M., Troyanovsky, S.M., Holland, R., van Haelst, U.J.G.M., Ramaekers, F.C.S. (1991). Basal cell-specific and hyperprolifeRation-related Keratins in Human breast cancer. Am. J. Pathol. 138, 751-63. Porter, R.M., Lunny, D.P., Ogden, P.H., Morley, S.M., McLean, W.H., Evans, A., Harrison, D.L., Rugg, E.L., Lane, E.B. (2000). K15 expression implies lateral differentiation within stRatified epithelial basal cells. Lab Invest. 80, 1701-10. Ahenkorah, A., Hottor, B., Byrne, S., Bosio, P. and Ockleford, C.D. (2008). Immunofluorescence confocal laser scanning microscopy and immuno-electron microscopic identifiCation of Keratins in Human materno-foetal interaction zone. J. Cell. Mol. Med. 13, 735-48. van Lingen, R.G., Poll, M.K.P., Seyger, M.M.B., de Jong, E.M.G.J., van de Kerkhof, P.C.M., van Erp, P.E.J. (2008). Distribution of peptidyl-peptidase IV on Keratinocytes in the margin zone of a psoriatic lesion: a comparison with hyperprolifeRation and aberrant differentiation markers. Arch Dermatol Res 300, 561-67. Poschmann, G., Sitek, B., Sipos, B., Ulrich, A., Wiese, S., Stephan, C., Warscheid, B., Klöppel, G., Vander Borght, A., Ramaekers, F.C.S., Meyer, E. and Stühler, K. (2009). IdentifiCation of proteomic differences between squamous cell carcinoma of the lung and bronchial epithelium. Molecular & Cellular proteomics 8, 1105-16.
• Protein number
  P08779
• Warnings
  This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This product contains sodium azide. To prevent formation of toxic vapors, do not mix with strong acidic solutions. To prevent formation of potentially explosive metallic azides in metal plumbing, always wash into drain with copious quantities of water. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
• Antibody s link
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
• Test
  Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
• Latin name
  Mus musculus

• Gene target
  Cytokeratin   Keratin   K16  
• Short name
  Mouse anti Cytokeratin 16 / Keratin K16
• Technique
  Mouse, anti, antibody to, mouses
• Host
  mouse
• Isotype
  IgG1
• Label
  unlabeled
• Species
  Mouse, Mouses
• Alternative name
  Mouse antibody to Cytokeratin 16 / Keratin K16
• Alternative technique
  murine, antibodies

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

Product images

Similar products