HB-EGF, human recombinant

• Catalog number
  4266-1000
• Price
  Please ask
• Size
  1 mg

• Synonyms
  Proheparin-binding EGF-like growth factor, HBEGF, DTR, DTS, HEGFL, HB-EGF, Heparin-binding EGF-like growth factor, Diphtheria toxin receptor, DT-R, DTSF.
• Alternative_names
  Proheparin-binding EGF-like growth factor, HBEGF, DTR, DTS, HEGFL, HB-EGF, Heparin-binding EGF-like growth factor, Diphtheria toxin receptor, DT-R, DTSF.
• Description
  EGF like growth factor involved in cell proliferation
• Recombinant
  Yes
• Source
  E. coli
• Purity by SDS PAGE
  ≥97%
• Assay
  SDS-PAGE
• Purity
  ≥97%
• Biological activity
  The ED50 was determined by a cell proliferation assay using balb/c 3T3 cells is < 1.0 ng/ml, corresponding to a specific activity of > 1.0×106 units/mg.
• Results
  > 1.0×106 units/mg.
• Molecular Weight
  9.7 kDa
• Storage Temp
  -20°C
• Shipping
  Gel pack
• Shelf Life
  12 months
• Appearance
  Lyophilized protein
• Physical form description
  Lyophilized with no additives
• Reconstitution Instructions
  It is recommended to reconstitute the lyophilized Human HB-EGF in sterile 18M-cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
• Background Information
  HB-EGF is an EGF related growth factor which signals via the EGF receptor, and stimulates the proliferation of SMC (smooth muscle cells), fibroblasts, epithelial cells and keratinocytes. HB-EGF is expressed in various cell types and tissues, including vascular endothelial cells and SMC, macrophages, skeletal muscle, keratinocytes and particular tumor cells. HB-EGF’s ability to explicitly bind heparin and heparin sulfate proteoglycans is dissimilar from other EGF-like molecules, and might be related to the enhanced mitogenic activity, relative to EGF, that HB-EGF exerts on smooth muscle cells.
• Amino acid sequence
  DLQEADLDLL RVTLSSKPQA LATPNKEEHG KRKKKGKGLG KKRDPCLRKY KDFCIHGECK YVKELRAPSC ICHPGYHGER CHGLSL.
• Handling
  Centrifuge the vial prior to opening.
• Usage
  For Research Use Only! Not to be used in humans
• Datasheet
• Properties
  Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Additional source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  HB-EGF  
• Gene symbol
  EGF, HMHB1, KRT84, HBEGF, KRT81, KRT85, KRT83, KRT82, PEG10
• Short name
  HB-EGF, recombinant
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
• Species
  Human, Humans
• Alternative name
  HB-epidermal growth factor, H. sapiens Rec.
• Alternative technique
  rec
• Alternative to gene target
  epidermal growth factor, HOMG4 and URG, EGF and IDBG-34100 and ENSG00000138798 and 1950, transmembrane receptor protein tyrosine kinase activator activity, Extracellular, Egf and IDBG-193432 and ENSMUSG00000028017 and 13645

Gene info

• Identity
  HGNC:3229
• Gene
  EGF
• Long gene name
  epidermal growth factor
• Synonyms gene name
  • epidermal growth factor (beta-urogastrone)
• Synonyms name
  • Pro-epidermal growth factor
• GenBank acession
  • X04571
• Locus
  4q25
• Discovery year
  2001-06-22
• Entrez gene record
  1950
• VEGA ID
  OTTHUMG00000132044

Gene info

• Identity
  HGNC:29677
• Gene
  HMHB1
• Long gene name
  histocompatibility minor HB-1
• Synonyms
  • HB-1
  • HB-1Y
• GenBank acession
  • AF103884
• Locus
  5q31.3
• Discovery year
  2006-07-20
• Entrez gene record
  57824
• Pubmed identfication
  • 9892612
• RefSeq identity
  • NM_021182
• Classification
  • Minor histocompatibility antigens
• VEGA ID
  OTTHUMG00000163173

Gene info

• Identity
  HGNC:6461
• Gene
  KRT84
• Long gene name
  keratin 84
• Synonyms gene
  • KRTHB4
• Synonyms gene name
  • keratin, hair, basic, 4
  • keratin 84, type II
• Synonyms
  • Hb-4
• Synonyms name
  • hard keratin type II 4
• GenBank acession
  • Y19209
• Locus
  12q13.13
• Discovery year
  1998-04-21
• Entrez gene record
  3890
• Pubmed identfication
  • 2431943
  • 16831889
• RefSeq identity
  • NM_033045
• Classification
  • Keratins, type II
• VEGA ID
  OTTHUMG00000169634

Gene info

• Identity
  HGNC:3059
• Gene
  HBEGF
• Long gene name
  heparin binding EGF like growth factor
• Synonyms gene
  • HEGFL
  • DTS
  • DTR
• Synonyms gene name
  • diphtheria toxin receptor (heparin-binding epidermal growth factor-like growth factor)
  • heparin-binding EGF-like growth factor
• Synonyms name
  • Diphtheria toxin receptor (heparin-binding EGF-like growth factor)
  • heparin-binding epidermal growth factor
• Locus
  5q31.3
• Discovery year
  2001-06-22
• Entrez gene record
  1839
• Pubmed identfication
  • 7590736
  • 12163414
• RefSeq identity
  • NM_001945
• VEGA ID
  OTTHUMG00000129496

Gene info

• Identity
  HGNC:6458
• Gene
  KRT81
• Long gene name
  keratin 81
• Synonyms gene
  • KRTHB1
• Synonyms gene name
  • keratin, hair, basic, 1
  • keratin 81, type II
• Synonyms
  • Hb-1
• Synonyms name
  • hard keratin type II 1
• GenBank acession
  • X81420
• Locus
  12q13.13
• Discovery year
  1998-04-21
• Entrez gene record
  3887
• Pubmed identfication
  • 7556444
  • 16831889
• RefSeq identity
  • NM_002281
• Classification
  • Keratins, type II
• VEGA ID
  OTTHUMG00000167574

Gene info

• Identity
  HGNC:6462
• Gene
  KRT85
• Long gene name
  keratin 85
• Synonyms gene
  • KRTHB5
• Synonyms gene name
  • keratin, hair, basic, 5
  • keratin 85, type II
• Synonyms
  • Hb-5
• Synonyms name
  • hard keratin type II
• GenBank acession
  • X99140
• Locus
  12q13.13
• Discovery year
  1998-04-21
• Entrez gene record
  3891
• Pubmed identfication
  • 9084137
  • 16831889
• RefSeq identity
  • NM_002283
• Classification
  • Keratins, type II
• VEGA ID
  OTTHUMG00000169633

Gene info

• Identity
  HGNC:6460
• Gene
  KRT83
• Long gene name
  keratin 83
• Synonyms gene
  • KRTHB3
• Synonyms gene name
  • keratin, hair, basic, 3
  • keratin 83, type II
• Synonyms
  • Hb-3
• Synonyms name
  • hard keratin type II
• GenBank acession
  • X99141
• Locus
  12q13.13
• Discovery year
  1998-04-21
• Entrez gene record
  3889
• Pubmed identfication
  • 9084137
  • 16831889
• RefSeq identity
  • NM_002282
• Classification
  • Keratins, type II
• VEGA ID
  OTTHUMG00000169632
• Locus Specific Databases
  • LRG_1302

Gene info

• Identity
  HGNC:6459
• Gene
  KRT82
• Long gene name
  keratin 82
• Synonyms gene
  • KRTHB2
• Synonyms gene name
  • keratin, hair, basic, 2
  • keratin 82, type II
• Synonyms
  • Hb-2
• Synonyms name
  • hard keratin type II 2
• GenBank acession
  • Y19207
• Locus
  12q13.13
• Discovery year
  1998-04-21
• Entrez gene record
  3888
• Pubmed identfication
  • 2431943
  • 16831889
• RefSeq identity
  • NM_033033
• Classification
  • Keratins, type II
• VEGA ID
  OTTHUMG00000169636

Gene info

• Identity
  HGNC:14005
• Gene
  PEG10
• Long gene name
  paternally expressed 10
• Synonyms
  • KIAA1051
  • HB-1
  • MEF3L
  • RGAG3
  • Mar2
  • Mart2
  • SIRH1
  • RTL2
• Synonyms name
  • Sushi-Ichi retrotransposon homolog 1
  • mammalian retrotransposon-derived 2
  • retrotransposon Gag like 2
• GenBank acession
  • AB049834
• Locus
  7q21.3
• Discovery year
  2000-11-28
• Entrez gene record
  23089
• Pubmed identfication
  • 11318613
  • 15716091
  • 16093683
• RefSeq identity
  • NM_015068
• Classification
  • Retrotransposon Gag like
• VEGA ID
  OTTHUMG00000155578
• Locus Specific Databases
  • LRG_1097

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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