Anti-LIMK1 Antibody

• Catalog number
  A2228-100
• Price
  Please ask
• Size
  100 µl

• Antibody Target
  LIMK1
• Target Alternative Name
  LIMK, LIM domain kinase 1, LIMK-1, EC 2.7.11.1, LIM Motif-Containing Protein Kinase, LIMK1
• Antibody Type
  Polyclonal
• Species Reactivities
  Rat,Mouse,Human
• Immunogen Sequence
  KLH-conjugated synthetic peptide targeting a sequence within the center region of human LIMK1
• Accession
  P53667
• Gene Id
  3984
• Appearance
  Colorless liquid
• Form
  Liquid
• Formulation
  In 0.42% Potassium phosphate; 0.87% NaCl; pH 7.3; 30% glycerol; and 0.01% sodium azide
• Purification
  Affinity purified
• Application
  Immunofluorescence (IF),Western Blotting
• Application And Usages
  WB 1:500 - 1:1000, IF 1:100 - 1:500
• Description
  LIMK1 is a serine-threonine protein kinase that contains 2 zinc finger motifs, known as LIM motifs at their N-terminus and a kinase sequence at their C terminus. The protein is expressed ubiquitously in different regions of the adult brain. It has reduced expression in the heart and skeletal muscles. LIMK1 prevents the cleavage of actin filament and stabilizes the actin cytoskeleton by phosphorylating and inactivating actin binding factors such as cofilin-1 (CFL1), cofilin-2 (CFL2), and destrin (DSTN). Additionally, it acts downstream of Rho family GTPase signal transduction pathways. Kinases such as ROCK1, PAK1, and PAK4 phosphorylate LIMK1 on the threonine residue in the activation loop and increase the activity of LIMK1. LIMK1 deletion is associated with the impairment of visuospatial cognition and long-term memory of Williams syndrome. Overexpression of LIMK1 is observed in multiple cancers such as breast, colorectal, and prostate cancer.
• Storage Condition
  -20ºC
• Shipping Condition
  Gel Pack
• Shelf Life
  12 months
• Usage
  For Research Use Only! Not to be used in humans.
• Handling
  The antibody solution should be gently mixed before use.
• Western Blot Verified
  1
• Immunofluorescence Verified
  1
• Data Sheet Link
• Additional description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
• Properties
  If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• French translation
  anticorps

• Gene target
  LIMK1  
• Gene symbol
  LIMK1
• Short name
  Anti-LIMK1 Antibody
• Technique
  Antibody, anti-, anti, antibody to, antibodies, antibodies against human proteins, antibodies for
• Isotype
  IgG
• Alternative name
  Antibody toLIMK1 (antibody to-)
• Alternative technique
  antibodies

Gene info

• Identity
  HGNC:6613
• Gene
  LIMK1
• Long gene name
  LIM domain kinase 1
• Synonyms
  • LIMK
• GenBank acession
  • D26309
• Locus
  7q11.23
• Discovery year
  1996-03-14
• Entrez gene record
  3984
• Pubmed identfication
  • 8673124
  • 8812460
• RefSeq identity
  • NM_002314
• Classification
  • LIM domain containing
  • PDZ domain containing
  • LIMK/TESK kinase family
• VEGA ID
  OTTHUMG00000023448

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

Product images

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