Soluble RANK Ligand Recombinant Protein
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Catalog number
40-481
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Price
Please ask
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Size
0.002 mg, 0.01 mg
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About recombinant protein
RANKL and RANK are members of the TNF superfamily of ligands and receptors that play an important role in the regulation of specific immunity and bone turnover. RANK (receptor) was originally identified as a dendritic-cell-membrane protein, which by interacting with RANKL augments the ability of dendritic cells to stimulate naïve T-cell proliferation in a mixed lymphocyte reaction, to promote the survival of RANK + T cells, and to regulate T-cell-dependent immune response. RANKL, which is expressed in a variety of cells including osteoblasts, fibroblasts, activated T-cells and bone marrow stromal cells, is also capable of interacting with a decoy receptor called OPG. Binding of soluble OPG to sRANKL inhibits osteoclastogenesis by interrupting the signaling between stromal cells and osteoclastic progenitor cells, thereby leading to excess accumulation of bone and cartilage. Recombinant rat sRANKL is a 19.4 kDa polypeptide comprising the TNF homologous region of RANKL (174 amino acid residues).
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Other name of the recombinant
RANKL, Opgl, Rankl, Trance, Tumor necrosis factor ligand superfamily member 11, Osteoclast differentiation factor, ODF
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Recombinant in
E. coli
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SEQUENCE
PAMMEGSWLD VARRGKPEAQ PFAHLTINAA DIPSGSHKVS LSSWYHDRGW AKISNMTLSN GKLRVNQDGF YYLYANICFR HHETSGSVPA DYLQLMVYVV KTSIKIPSSH NLMKGGSTKN WSGNSEFHFY SINVGGFFKL RAGEEISVQV SNPSLLDPDQ DATYFGAFKV QDID
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Activity
Biological activity was determined by its ability to induce osteoclast formation in RAW264.7 cells using a concentration range of 5.0 - 10.0 ng/mL.
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Keep at
The lyophilized Soluble RANK Ligand recombinant protein is stable for at least 2 years from date of receipt at -20˚C. Reconstituted Soluble RANK Ligand is stable for at least 3 months when stored in working aliquots with a carrier protein at -20˚C. As with any protein, exposing Soluble RANK Ligand recombinant protein to repeated freeze / thaw cycles is not recommended. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.
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Rec PROTEIN GI
16924012
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Accession nr
NP_476490.1
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Pureity
Greater than 98% by SDS-PAGE gel and HPLC analyses.Endotoxin level is less than 0.1 ng per μg (1EU/μg).
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About
This product is for research use only.
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Protein s origin
Rat
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Vial
Lyophilized
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NCBI ID
117516
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Gene name
tumor necrosis factor (ligand) superfamily, member 11
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Source
Rattus norvegicus
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Uniprot
Q9ESE2
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Description
FAS ligand and other ligands are binding to the receptor for signaling pathways for example in apoptosis or JNK signaling. Receptor agonists are often tested for drug development.
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Additional source
Recombinants or rec. proteins
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Group
recombinants
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Gene target
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Gene symbol
Tnfsf11
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Short name
Soluble RANK Ligand Recombinant Protein
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Technique
Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. proscience advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
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Alternative name
Soluble RANK Ligand Rec. Protein
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Alternative technique
rec
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Gene info
MeSH Data
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Name
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Concept
Scope note:
The initial culturing of cells derived directly from fresh TISSUES.
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Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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