-
Category
Primary Antibodies
-
Long description
The CD36 molecule is an 88kDa transmembrane glycoprotein, which is a member of the class B scavenger receptor family of proteins. Alternative names for CD36 include fatty acid translocase (FAT), platelet glycoprotein 4, platelet glycoprotein IIIb and thrombospondin receptor. CD36 is a pattern recognition receptor that is expressed on a wide variety of cells including monocytes, macrophages, platelets, adipocytes, erythrocyte precursors, activated keratinocytes and some endothelial and epithelial cells. Studies suggest that CD36 has multiple cellular functions and binds to a diverse array of ligands including thrombospondin, modified low-density lipoproteins (LDL), anionic phospholipids and long chain fatty acids. As a scavenger receptor, CD36 plays a key role in mediating the uptake of oxidized LDL by macrophages, the formation of foam cells and the development of atherosclerosis. CD36 is also reported to function as negative regulator of angiogenesis, as a cell adhesion molecule involved in platelet adhesion and aggregation, and as a scavenger receptor in the clearance of apoptotic cells and photoreceptor rod outer segments. Furthermore, CD36 is reported to bind to Plasmodium falciparum infected erythrocytes and may play an important role in the pathogenesis of malaria and sickle cell disease.
-
Antibody come from
185-1G2 is a mouse monoclonal IgG2a antibody derived by fusion of NS1 mouse myeloma cells with spleen cells from BALB/c mice immunized with stimulated human leucocytes.
-
Other description
Each vial contains 100µl 1mg/ml purified monoclonal antibody in phosphate buffered saline (PBS) containing 0.09% sodium azide. This product is lyophilized
-
Clone
185-1G2
-
Antigen antibody binding interaction
Mouse anti Human CD36 Antibody
-
Antibody is raised in
Mouse
-
Antibody s reacts with
Human
-
Antibody s reacts with these species
This antibody doesn't cross react with other species
-
Antibody s specificity
The mouse monoclonal antibody 185-1G2 recognises human CD36. The antibody recognises an epitope between amino acids 155 – 183 on the CD36
-
Research interest
Immunology, cell adhesion
-
Application
Immunocytochemistry, Immunohistochemistry (Frozen), Flow Cytometry
-
Antibody s suited for
The antibody 185-1G2 can be used for CD36 cell identification, quantification and isolation using flow cytometric methods. The antibody is suitable for use in immunocytochemistry and immunohistochemistry on frozen tissue sections. The antibody has also been reported to bind to a site of CD36 that inhibits CD36 function. For this application, please request a sodium azide free antibody format. Optimal antibody dilutions for the different applications should be determined by titration. The recommended range for flow cytometry is 1:100 – 1:200.
-
Storage
Store at 4°C, or in small aliquots at -20°C.
-
Relevant references
1. Howard LM, Puente Navazo MD, McGregor JL. Identification of CD36-ractive antibodies that bind an epitope encompassing amino acid residues 155-183. In Leucocyte typing VI. Sixth International workshop and conference. Kishimoto T, Kikutani H, von dem Borne AEG.Kr, Goyert SA, Mason DY, Miyasaka M, Moretta L, Okumura K, Shaw S, Springer TA, Sugamura K, Zola H. Eds. Kobe, Japan, Garland. 1996. p637-638. 2. Englyst NA, Taube JM, Aitman TJ, Baglin TP, Byrne CD. A novel role for CD36 in VLDL-enhanced platelet adhesion. Diabetes 2003; 52:1248 – 1255.
-
Protein number
P16671
-
Warnings
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This product contains sodium azide. To prevent formation of toxic vapors, do not mix with strong acidic solutions. To prevent formation of potentially explosive metallic azides in metal plumbing, always wash into drain with copious quantities of water. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
-
-
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody against the Hu protein or peptide or recombinant supplied in 1 volumes. Ask for quote if you need bulk.
-
Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
-
Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
-
Latin name
Mus musculus
