Anti-Dnmt1 (Polyclonal), ALEXA Fluor 594

• Catalog number
  GENTObs-0678R-A594
• Price
  Please ask
• Size
  100 microliters

• Type
  Conjugated Primary Antibody
• Conjugated with
  ALEXA FLUOR® 594
• Host organism
  Rabbit (Oryctolagus cuniculus)
• Target Protein Peptide
  Dnmt1
• Specificity
  This antibody will not recongize isoform 3.Dnmt1
• Modification
  No modification has been applied to this antibody
• Modification site
  None
• Clonality
  Polyclonal Antibody
• Clone
  Polyclonal Antibodies
• Concentration
  1ug per 1ul
• Subcellular locations
  Nucleus
• Antigen Source
  KLH conjugated synthetic peptide derived from human Dnmt1
• Gene ID
  1786
• Swiss Prot
  P26358
• Applications
  IF(IHC-P)
• Applications with corresponding dilutions
  IF(IHC-P)(1:50-200)
• Cross reactive species
  Human (Homo sapiens), Mouse (Mus musculus), Rat (Rattus norvegicus)
• Cross Reactive Species details
  No significant cross reactivity has been observed for this antibody for the tested species. However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species.
• Background information
  Methylates CpG residues. Preferentially methylates hemimethylated DNA. Associates with DNA replication sites in S phase maintaining the methylation pattern in the newly synthesized strand, that is essential for epigenetic inheritance. Associates with chromatin during G2 and M phases to maintain DNA methylation independently of replication. It is responsible for maintaining methylation patterns established in development. DNA methylation is coordinated with methylation of histones. Mediates transcriptional repression by direct binding to HDAC2. In association with DNMT3B and via the recruitment of CTCFL/BORIS, involved in activation of BAG1 gene expression by modulating dimethylation of promoter histone H3 at H3K4 and H3K9.
• Purification method
  Purified by Protein A.
• Storage
  Water buffered solution containing 100ug/ml BSA, 50% glycerol and 0.09% sodium azide. Store at 4°C for 12 months.
• Excitation emission
  590nm/617nm
• Synonyms
  AIM; DNMT; MCMT; CXXC9; HSN1E; ADCADN; DNA (cytosine-5)-methyltransferase 1; Dnmt1; CXXC-type zinc finger protein 9; DNA methyltransferase HsaI; DNA MTase HsaI; M.HsaI
• Also known as
  Dnmt1 Polyclonal Antibody
• Other name
  Anti-Dnmt1 Polyclonal
• Advisory
  Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody, thus reducing its reactivity, specificity and sensitivity. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.
• Properties
  For facs or microscopy Alexa 1 conjugate.
• Conjugation
  Alexa Fluor, ALEXA FLUOR® 594
• Conjugated
  Alexa conjugate 1
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
• Group
  Polyclonals and antibodies
• About
  Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.

• Gene target
  Dnmt1  
• Gene symbol
  DALIR, DACOR1, DNMT1
• Short name
  Anti-Dnmt1
• Technique
  Polyclonal, anti-, anti, antibody to, antibodies, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
• Isotype
  Immunoglobulin G (IgG)
• Label
  ALEXA FLUOR 594
• Alternative name
  Dnmt1 Polyclonal Antibody, ALEXA FLUOR® 594 Conjugated
• Alternative technique
  polyclonals, antibodies

Gene info

• Identity
  HGNC:51429
• Gene
  DALIR
• Long gene name
  DNMT1-associated long intergenic non-coding RNA
• Synonyms
  • DALI
• Synonyms name
  • DNMT1-Associated Long Intergenic
• GenBank acession
  • BE439524
  • DA337013
  • EB386236
• Locus
  2q12
• Discovery year
  2014-12-10
• Pubmed identfication
  • 25415054
• Classification
  • Long non-coding RNAs with non-systematic symbols

Gene info

• Identity
  HGNC:51902
• Gene
  DACOR1
• Long gene name
  DNMT1-associated colon cancer repressed lncRNA 1
• Synonyms
  • TCONS_00023265
• Locus
  15q22.33
• Discovery year
  2015-09-18
• Entrez gene record
  100131796
• Pubmed identfication
  • 26307088
• Classification
  • Long non-coding RNAs with non-systematic symbols

Gene info

• Identity
  HGNC:2976
• Gene
  DNMT1
• Long gene name
  DNA methyltransferase 1
• Synonyms gene
  • DNMT
• Synonyms gene name
  • DNA (cytosine-5-)-methyltransferase 1
• Synonyms
  • MCMT
  • CXXC9
• GenBank acession
  • X63692
• Locus
  19p13.2
• Discovery year
  1991-06-04
• Entrez gene record
  1786
• Pubmed identfication
  • 1594447
• RefSeq identity
  • NM_001379
• Classification
  • 7BS C5-cytosine DNA/RNA methyltransferases
  • Zinc fingers CXXC-type
• VEGA ID
  OTTHUMG00000180397
• Locus Specific Databases
  • LRG_362

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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