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Available ordering format
Liquid at 23.3 µg/µl in PBS pH 8.0 + 0.02% sodium azide as preservative
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Immunogen
KLH-conjugated synthetic peptide derived from available plant, algal (chloroplastic and mitochondrial) and bacterial sequences of beta subunits of F-type ATP synthases, including Arabidopsis thaliana chloroplastic ATP synthase subunit beta UniProt: P19366, TAIR: AtCg00480 and Arabidopsis thaliana mitochondrial ATP synthase subunit beta-1, UniProt: P83483, TAIR: At5g08670 as well as Chlamydomonas reinhardtii, UniProt: P06541 and A8IQU3
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Raised in
Chicken
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Clonality
Polyclonal
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Clone
Polyclonal
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Purification
Total IgY
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How to reconstitute
See included datasheet or contact our support service
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Storage condition
store at 4°C; make aliquots to avoid working with a stock. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from liquid material adhering to the cap or sides of the tubes.
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Verified applications
Western blot (WB), immunolocalization (IL)
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Connected products
AS05 085 | anti-ATP synthase subunit beta, rabbit antibodyAS03 030S | ATP synthase subunit beta protein standard for quantitation and positive controlAS08 304 | anti-ATP synthase subunit alpha, rabbit antibodyAS08 312 | anti-ATP synthase subunit gamma, rabbit antibodyAS05 071 | anti-ATP synthase subunit c, rabbit antibodycollection of antibodies to proteins involved in bioenergetics of a plant cellPlant and algal protein extraction bufferSecondary antibodies
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Recommended dilutions for use
1: 5 000 - 1: 8 000 (WB), 1: 500 for localization of native enzyme by immunogold (IL)
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Molecular weight expected аpparent
53.9 kDa (Arabidopsis thaliana), 51.7 kDa (Synechocystis PCC 6803), 53.7 kDa (Spinacia oleracea)
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Verified reactivity
Arabidopsis thaliana, Hordeum vulgare, Spartina alterniflora, Spinacia oleracea, Synechocystis PCC 6803, Synechococcus PCC 7942, beef muscle, rat liver
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Possible reactivity
dicots including Glycine max, Vitis vinifera and monocots including Oryza sativa, Chlamydomonas reinhardtii, cyanobacteria, marine diatoms, Acinetobacter baumannii, Clostridium sp., bacteria including Yrsinia sp.
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No reactivity
archeal V-type ATP synthase
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Supplementary information
results of immunogold studies using anti-AtpB antibody are published in Andersson et al. (2009)
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References
Quesada et al. (2011). Arabidopsis RUGOSA2 encodes an mTERF family member required for mitochondrion, chloroplast and leaf development. Plant J. Nov;68(4):738-53. doi: 10.1111/j.1365-313X.2011.04726.x. Epub 2011 Sep 13.Andersson et. al (2009). Co-localization of P-glycerate kinase, P-ribulokinase, ADP-glucose pyrophosphorylase and Rubisco activase with CF1 in pea leaf chloroplasts. Plant Science 177:136-14. (immunolocalization)Morash et al. (2007). Macromolecular dynamics of the photosynthetic system over a seasonal developmental progression in Spartina alterniflora. Canadian J. of Botany, 2007, 85(5): 476-483, 10.1139/B07-043.
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Scientific context
ATP synthase is the universal enzyme that synthesizes ATP from ADP and phosphate using the energy stored in a transmembrane ion gradient.
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Notes
The anti-AtpB antibody will detect the mitochondrial form of the F1 ATP synthase subcomplex, as well as the chloroplastic CF1 ATP synthase and most known bacterial F-type ATP synthases. Peptide used for antibody production is located in a beta sheet, which is partly exposed near the surface of the AtpB protein.
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Protein number
P19366 , P83483 , P06541 , A8IQU3
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TAIR number
AT5G08670, ATCG00480
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