Anti-ACTA2 Rabbit Monoclonal Antibody

  • Catalog number
    A1118-50
  • Price
    Please ask
  • Size
    50 µl
  • Antibody Target
    ACTA2
  • Target Alternative Name
    alpha smooth muscle Actin Alpha-actin-2, Cell growth-inhibiting gene 46 protein
  • Antibody Type
    Monoclonal
  • Clone
    RM253
  • Species Reactivities
    Human and mouse
  • Immunogen Sequence
    A synthetic peptide corresponding to the N-terminus of human alpha smooth muscle Actin
  • Accession
    P62736
  • Gene Id
    59
  • Appearance
    Colorless liquid
  • Form
    Liquid
  • Formulation
    In 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
  • Purification
    Affinity purified using protein A
  • Application
    IHC, WB
  • Positive Control
    WB: heart tissue lysate, IHC: kidney and heart tissue sections
  • Application And Usages
    IHC: 1:1000 -1:2500 dilution; WB: 1:1000 - 1:2000 dilution
  • Description
    Actins are highly conserved proteins expressed in all eucaryotic cells. Actin filaments form part of the cytoskeleton and play essential roles in regulating cell shape and movement. Six distinct actin isotypes have been identified in mammalian cells. Each is encoded by a separated gene and is expressed in a developmentally regulated and tissue-specific manner, alpha and beta cytoplasmic actins are expressed in a wide variety of cells; whereas, expression of alpha skeletal, alpha cardiac, alpha vascular, and gamma enteric actins are more restricted to specialized muscle cell type. Smooth muscle alpha actin is of further interest because it is one of a few genes whose expression is relatively restricted to vascular smooth muscle cells. Furthermore, expression of smooth muscle alpha actin is regulated by hormones, cell proliferation, and altered by pathological conditions including oncogenic transformation and atherosclerosis.
  • Storage Condition
    -20 °C
  • Shipping Condition
    gel pack
  • Shelf Life
    12 months
  • Usage
    For Research Use Only! Not to be used in humans.
  • Handling
    The antibody solution should be gently mixed before use.
  • Western Blot Verified
    Verified
  • Immunocytochemistry Verified
    Verified
  • Immunofluorescence Verified
    Not Verified
  • Immunoprecipitation Verified
    Not Verified
  • FACS Verified
    Not Verified
  • ELISA Verified
    Not Verified
  • ChIP Verified
    Not Verified
  • Dot Blot Verified
    Not Verified
  • Flow Cytometry Verified
    Not Verified
  • Additional description
    This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
  • Properties
    If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
  • About
    Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes. Rabbits are used for polyclonal antibody production by Biovision. Rabbit antibodies are very stable and can be stored for several days at room temperature. Biovision adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
  • Latin name
    Oryctolagus cuniculus
  • French translation
    anticorps
  • Gene target
  • Gene symbol
    ACTA2, ACTA2-AS1
  • Short name
    Anti-ACTA2 Rabbit Monoclonal Antibody
  • Technique
    Antibody, Rabbit, anti-, anti, antibody to, antibodies, antibodies against human proteins, antibodies for, Monoclonals or monoclonal antibodies
  • Host
    Rabbit, Rabbits
  • Isotype
    Rabbit IgG
  • Alternative name
    Antibody toACTA2 production species: rabbit monoclonal (antibody to-)
  • Alternative technique
    antibodies, rabbit-anti
Gene info
Gene info
MeSH Data
  • Name
  • Concept
    Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
  • Tree numbers
    • E05.196.401.143
    • E05.301.300.096
    • E05.478.566.320.200
    • E05.601.262
    • E05.601.470.320.200
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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